Novel detection kit for copy number of fibroblast growth factor 19 (FGF19) gene

Abstract

The invention relates to a detection kit for the copy number of a fibroblast growth factor 19 (FGF19) gene, and belongs to the molecular biology technical field; the kit is composed of a fluorescent quantitative reaction liquid pre-mixed solution, FGF19 target gene sequence PCR amplification primers and internal reference sequence PCR amplification primers. A detection method includes the steps: a sequence on an exon 2 of the FGF19 gene is taken as a template for designing the detection primers, a set of internal reference sequence amplification primers (MRef) is designed in view of a segment of stable sequence randomly uniformly distributed and appearing more than 40 times in a human genome, a target sequence is amplified by using a fluorescent quantitative PCR method, and resulting data is analyzed with the help of a set of on-line analysis software, so as to determine the copy number of the FGF19 gene in different samples. The detection kit has the advantages of high sensitivity, good specificity, and large dynamic range, and the detection method is simple, convenient and quick, and the detection kit can be applied to quantitative detection of the copy number of the FGF19 gene in various tissues, cells and blood samples.

Description

technical field [0001] The invention relates to a detection kit for the copy number of fibroblast growth factor 19 gene and a corresponding method, belonging to the technical field of molecular biology. Background technique [0002] Fibroblast growth factor 19 (Fibroblast growth factor 19, FGF19) gene is located on chromosome 11, about 6.1 kb in length, and consists of three exons and two introns. The amino acid sequence of the FGF19 gene is similar to the mouse FGF15 gene, and it can specifically combine with the fibroblast growth factor receptor 4 (FGFR4) gene. [0003] The binding of FGF19 to FGFR4 in hepatocytes can regulate bile synthesis by inhibiting the cholesterol 7α-hydroxylase (CYP7A1) gene, and in high-fat diet and leptin-deficient mice, FGF19 can also increase metabolic rate, reduce body weight and Reverse diabetes. Ectopic expression of FGF19 in mice promotes hepatocyte proliferation, hepatocyte dysplasia, and tumor formation. Studies have found that FGF19 i...

AI Technical Summary

Problems solved by technology

[0004] Fluorescence in situ hybridization (FISH) technology, which is commonly used clinically to detect gene copy number, can directly observe gene copy number changes and chromosomal abnormalities under a microscope, but it is time-consuming, laborious, and costly. High personnel requirements and other shortcomings; in the research field, high-throughput microarray ch

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