Screening method of Anhui local pig breed meat quality trait candidate genes

A meat quality trait, candidate gene technology, applied in the field of molecular biology, can solve problems such as lack of attention

Inactive Publication Date: 2015-09-16
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This is due to the long-term one-sided pursuit of fast growth and high lean meat percentage, and lack of attention to the improvement of...

Method used

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  • Screening method of Anhui local pig breed meat quality trait candidate genes
  • Screening method of Anhui local pig breed meat quality trait candidate genes
  • Screening method of Anhui local pig breed meat quality trait candidate genes

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] 1. Materials and methods

[0041] 1. Feeding and handling of experimental animals

[0042] 21 commercial pigs to be slaughtered were selected from the Wei pig breeding farm of Anhui Antai Agricultural Development Co., Ltd., 13 Anqing Liubai pigs were selected by Wangjiang County Modern Fine Breeding Co., Ltd., and 6 Landrace pigs were selected by Anhui Changfeng Agriculture and Animal Husbandry Technology Co., Ltd. According to the conventional feeding standard of each pig breed in the farm, it will be fed and managed (the slaughter date is 10 months for local pigs, and 6 months for Landrace pigs). Feed for 24 hours before slaughter, weighed, and slaughtered according to the "Technical Specifications for Measuring Carcass Traits of Lean Meat Pigs (NY / T825-2004)".

[0043] 2. Sample collection

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Abstract

The invention discloses a screening method of Anhui local pig breed meat quality trait candidate genes. The screening method comprises following steps: (1) selection of reference animal models; (2) sample collection, wherein after pig slaughter, longissimus dorsi, heart, and liver tissues are collected; (3) conventional meat quality index determination, wherein meat color, pH value, and tenderness of longissimus dorsi are determined; (4) determination of longissimus dorsi IMP content via chromatography; (5) determination of longissimus dorsi IMF content via Soxhlet extraction; and (6) determination of expression level of key genes in pig liver, heart, and longissimus dorsi in IMP and IMF forming processes via RT-PCR, and data statistic analysis. The screening method is simple in operation, low in cost, and ideal in detection results.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for screening candidate genes for meat quality traits of local Anhui pig breeds. Background technique [0002] With the improvement of living standards and the transformation of nutritional concepts, people have higher and higher requirements for pork quality. At present, improving pork quality has become the primary task in pig production. Over the past few decades, the hog industry has catered to market demand and devoted itself to the efficient production of lean-meat commercial pigs, which has shortened the growth cycle of commercial pigs and increased lean meat production; Inferior, the taste of pork is reduced, such as: rough, lack of meat flavor and aroma. This is due to the long-term one-sided pursuit of fast growth and high lean meat percentage, and lack of attention to the improvement of pork quality and the genetic basis of meat quality traits. This issue has...

Claims

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Application Information

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IPC IPC(8): G01N3/24G01N1/04G01N30/06C12Q1/68
Inventor 丁月云殷宗俊周杰张淑静刘力源张晓东朱宏宇黄龙吴涛张威
Owner ANHUI AGRICULTURAL UNIVERSITY
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