Application of radix acanthopanacis trifoliate extract in inhibiting alpha-glucosidase

A technology of glucosidase and extract, which is applied in the field of application of bok choy extract to inhibit α-glucosidase, and can solve problems such as no blood sugar lowering effect of bok choy

Active Publication Date: 2015-09-23
WUYI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no research reports on the hypoglycemic effect of bougainvillea

Method used

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  • Application of radix acanthopanacis trifoliate extract in inhibiting alpha-glucosidase
  • Application of radix acanthopanacis trifoliate extract in inhibiting alpha-glucosidase
  • Application of radix acanthopanacis trifoliate extract in inhibiting alpha-glucosidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of bok choy extract

[0030] Collect fresh leaves, stems, and roots of bougainvillea, dry them at room temperature, crush them, take out 200g each, add 2L 95% edible alcohol for cold extraction, filter every other day, extract three times in total, combine the extracts, and concentrate them with a rotary evaporator The ethanol extracts of different parts (leaf: L; stem: S; root: R) were obtained. Each ethanol extract was sequentially extracted with petroleum ether, ethyl acetate and n-butanol, concentrated and dried to obtain petroleum ether layer (leaf: LP; stem: SP; root: RP), ethyl acetate layer (leaf: LE; stem: RP). : SE; root: RE), n-butanol layer (leaf: LN; stem: SN; root: RN). All extracts were vacuum-dried and stored at 4°C for future use.

Embodiment 2

[0032] Chemical Constituents of Bougainvillea Extract

[0033] The extract of the ethyl acetate layer of bok choy was separated and purified by repeated silica gel column chromatography, Sephadex LH-20 column chromatography, reversed phase silica gel column chromatography, preparative thin layer chromatography (pTLC), MCI resin column chromatography and other separation methods. To obtain monomer compounds, comprehensively use modern spectrum techniques (IR, UV, MS, 1 H-NMR, 13 C-NMR, DEPT, 1 H- 1 H COZY, HSQC, HMBC) and compared with the literature, the structures of 9 known terpenoids were determined, and their chemical names were impressive acid (1); 3α,11α-dihydroxy-lup-20(29)- en-23-al-28-oic acid(2); 3α-hydroxy-lup-20(29)-en-23,28-dioic acid(3); 3α,11α-dihydroxy-lup-20(29)- en-23,28-dioic acid(4); 3α-hydroxy-lup-20(29)-en-30-ol-23,28-dioic acid(5); ent-kaur-15-en-17-al -19-oic acid (6); 17-hydroxy-16α-ent-kauran-19-oic acid (7); 16α-hydroxy-ent-kauran-19-oic acid (8...

Embodiment 3

[0036] Inhibitory activity of bok choy extract on α-glucosidase

[0037]Preparation of α-glucosidase solution: Dissolve the lyophilized enzyme powder in 0.1% BSA solution to prepare 100 U / mL enzyme solution, and store it in a refrigerator at -20°C. Before the experiment, pipette a small amount of 100U / mL enzyme solution, first dilute to 20U / mL with 0.1% BSA solution, and then dilute to 0.2U / mL with 0.1% BSA solution for use.

[0038] Preparation of PNPG solution: Weigh a certain amount of PNPG solid, dissolve it with 0.1mol / L pH 6.8 phosphate buffer saline (PBS) to make a concentration of 10mmol / L, and then dispense it into 1.5mL centrifuge tubes.

[0039] In this experiment, the activity of α-glucosidase was determined by colorimetric method. The bok choy extract or compound to be tested was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution with a mass concentration of 10 mg / mL. Then dilute the stock solution with PBS to prepare the solution to be tested at...

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Abstract

The invention discloses an application of radix acanthopanacis trifoliate extract in inhibiting alpha-glucosidase. The radix acanthopanacis trifoliate extract is extracted and concentrated by ethanol and then extracted respectively by petroleum ether, ethylacetate and normal butanol. The radix acanthopanacis trifoliate extract is identified to have 9 terpenoids. The invention also discloses an alpha-glucosidase inhibitor, and the alpha-glucosidase inhibitor comprises radix acanthopanacis trifoliate extract as well as one or more additives acceptable on the medicine. The experiment shows that the extract of radix acanthopanacis trifoliate leaves, stalks and roots has inhibitory activity for the alpha-glucosidase; in 9 terpenoids of the radix acanthopanacis trifoliate extract, the compound 6 has best activity for inhibiting the alpha-glucosidase, which is far higher than a reference product acarbose.

Description

technical field [0001] The invention relates to a new application of bok choy extract, in particular to the application of bok choy extract to prepare alpha-glucosidase inhibitors. Background technique [0002] Diabetes is a metabolic disease characterized by prolonged periods of elevated blood sugar levels. High blood sugar will cause three symptoms: eat more, drink more, urinate more, and lose weight. If left untreated, diabetes can lead to many complications. Acute complications include diabetic ketoacidemia and hyperosmolar hyperglycemic non-ketoacid coma; serious long-term complications include cardiovascular disease, stroke, chronic kidney disease, diabetic foot, and retinopathy. [0003] The World Health Organization's 2011 report pointed out that 346 million people worldwide suffer from diabetes, and an estimated 3.4 million people died of hyperglycemia in 2004, and more than 80% of diabetes deaths occurred in low- and middle-income countries. In 2013, there were ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/254A61P3/10
Inventor 张焜李冬利杜志云郑希
Owner WUYI UNIV
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