Unlock instant, AI-driven research and patent intelligence for your innovation.

Adjustment method of gradient delay volume

A volume adjustment and gradient delay technology, applied in the field of high performance liquid chromatography, which can solve the problems of high cost and inconvenience

Active Publication Date: 2017-09-26
DIONEX SOFTRON
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this solution can be inconvenient due to the size of the mixer and is also cost intensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Adjustment method of gradient delay volume
  • Adjustment method of gradient delay volume
  • Adjustment method of gradient delay volume

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] Such as figure 1 The graph shown shows how the change in GDV affects the analysis with respect to time at the (sample) injection at time 0 minutes. In the graph, the solvent composition is represented by a dotted line with respect to time. Solvent composition (several transitions in the form of inflection points) from a constant (lower or front horizontal section) lower limit (40% acetonitrile) through a changing (from 40% to 70% acetonitrile) composition or gradient (ascending linear central region) Change to a constant (upper or rear horizontal fraction) upper limit (acetonitrile 70%). As a gradient, for example, a gradient of increasing solvent component B, eg acetonitrile CAN, from 40% to 70% in 16 seconds (and a corresponding decrease of solvent component A or from 60% to 30% of the remaining solvent components) is used. In the upper graph (case A), the preset pump volume V of the volume regulator A = 25 μL, i.e., GDV increased by 25 μL, while in the lower graph...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The method involves determining value of a gradient delay volume in a liquid chromatography plant. The value is adjusted in a region of a volume of a volume adjusting device (5), when the value of the liquid chromatography plant departs. The value is adjusted or altered during and / or after a sample injection. Flow rate and / or pressure within the region of the liquid chromatography plant are maintained constant during alteration of the value by controlling opposite directions of a pump by a control device (60). An independent claim is also included for a sample transmitter.

Description

technical field [0001] The present invention is in the field of liquid chromatography, especially high performance liquid chromatography (HPLC). Liquid chromatography (especially HPLC) is used to separate the components of a sample liquid by means of a chromatographic column. In this case, the separation efficiency of the (separation) column depends inter alia on its length and the particle size of the packing material. For the best possible separation, a column of sufficient length and small particle size is required. Such columns have a high liquid resistance and therefore require much higher pressures than conventional columns in order to operate. [0002] In addition, a sufficiently fast analysis to allow high sample throughput is desired. This requires high flow rates in the column, thereby also increasing the back pressure of the column. Background technique [0003] One possible way to speed up the separation or increase the separation efficiency is to change the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/34
CPCB01D15/166G01N30/34
Inventor 赫尔曼·霍克格雷伯托马斯·瓦辛格
Owner DIONEX SOFTRON