Traditional Chinese medicine composition for preventing and treating honeybee microsporidiosis and use thereof

A technology for microsporidiosis and a composition is applied in the field of traditional Chinese medicine preparations to achieve the effects of reducing toxicity, low cost and wide sources

Active Publication Date: 2015-09-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combine the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh 10 g of Polygonum cuspidatum and Toosendan after drying at room temperature, respectively add 100 ml of cold water, completely immerse the two kinds of Chinese herbal medicines, let stand at room temperature for 2 h, first boil with high heat, then simmer for 10-20 minutes. After min, filter to get the filtrate. Then add 100 ml of cold water to the filtered residue, repeat the above frying steps, take the filtrate, combine the two filtrates, and store them in a sealed seal at low temperature. The aqueous extract of Polygonum cuspidatum and the aqueous extract of Toosendan are mixed in equal volumes with sucrose syrup with a concentration of 60% by mass to prepare a drug-containing syrup with a concentration of 4%, allowing the bees infected with Microsporidia to eat freely.

[0016] Results: On the 7th day and 13th day after infection, the number of Microsporidium spores in the infected bees fed the syrup containing the drug was 50% and 80% of those in the infecte...

Embodiment 2

[0018] Weigh 10 g of Polygonum cuspidatum and Toosendan after drying at room temperature, respectively add 100 ml of cold water, completely immerse the two kinds of Chinese herbal medicines, let stand at room temperature for 2 h, first boil with high heat, then simmer for 10-20 minutes. After min, filter to get the filtrate. Then add 100 ml of cold water to the filtered residue, repeat the above frying steps, take the filtrate, combine the two filtrates, and store them in a sealed seal at low temperature. Polygonum cuspidatum water extract and Toosendan water extract were mixed in equal volumes with sucrose syrup with a mass percentage concentration of 60% to prepare a drug-containing syrup with a concentration of 7% by volume, allowing bees infected with Microsporidia to feed freely.

[0019] Results: On the 7th day and 13th day after infection, the number of Microsporidia spores in the infected bees fed the syrup containing the drug was 33.5% and 35.4% of those in the infect...

Embodiment 3

[0021] Weigh 10 g of Polygonum cuspidatum and Toosendan after drying at room temperature, respectively add 100 ml of cold water, completely immerse the two kinds of Chinese herbal medicines, let stand at room temperature for 2 hours, first boil with high heat and then simmer for 40-60 minutes. After min, filter to get the filtrate. Then add 100 ml of cold water to the filtered residue, repeat the above frying steps, take the filtrate, combine the two filtrates, and store them in a sealed seal at low temperature. Polygonum cuspidatum water extract and Toosendan water extract are mixed in equal volumes with sucrose syrup with a mass percentage concentration of 60% to prepare a drug-containing syrup with a concentration of 13% by volume, so that bees infected with Microsporidia can feed freely.

[0022] Results: On the 7th day and 13th day after infection, the number of Microsporidium spores in the infected bees fed the medicated syrup was 40% and 50% of that in the infected bees...

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Abstract

The invention provides a traditional Chinese medicine composition for preventing and treating honeybee microsporidiosis. The traditional Chinese medicine composition comprises polygonum cuspidatum and szechwan chinaberry fruit aqueous extract products according to a volume ratio of 1: 1. The traditional Chinese medicine composition and sugar syrup with a concentration of 60-70% by mass are processed to form medicine-containing syrup with a concentration of 4-13% according to a certain volume ratio, and the medicine-containing syrup is freely eaten by honeybees suffered from honeybee microsporidiosis. Through action on honeybees and microsporidia, the traditional Chinese medicine composition can prevent and treat honeybee microsporidiosis. The traditional Chinese medicine composition reduces toxicity of szechwan chinaberry fruit in a single agent, has parasite expelling effects of szechwan chinaberry fruit and anti-inflammation and detumescence effects of polygonum cuspidatum and has basically no toxicity. The traditional Chinese medicine composition utilizes easily available medical raw materials with a wide source, can be prepared simply and has a low cost. The traditional Chinese medicine composition does not produce drug residue on honeybee products and the environment and does not pollute human and animals and the environment. The traditional Chinese medicine composition can be used in drugs for prevention and treatment on honeybee microsporidiosis.

Description

technical field [0001] The invention belongs to the technical fields of extraction methods of traditional Chinese medicines and traditional Chinese medicine preparations. Specifically, it relates to a traditional Chinese medicine combination for preventing and treating bee microsporidiosis, a preparation method and an application thereof. Background technique [0002] Honey bee microsporidiosis, also known as "bee microsporidiosis", is a common digestive tract infectious disease of adult bees caused by honey bee microsporidiosis. Microsporidium honeybee mainly infects the intestinal epithelial cells of adult bees, destroys the normal digestive function of bee intestines, reduces the immunity of bees, changes the collection behavior of bees, and shortens the life span of bees. When the bee colony is mildly ill, the symptoms are not obvious and are often ignored. And once severe, it may even cause the collapse of the entire bee colony. Microsporidia of honeybees are mainly ...

Claims

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Application Information

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IPC IPC(8): A61K36/704A61P1/00A61P33/00
CPCY02A50/30
Inventor 郑火青陈秀贤胡福良张翠平
Owner ZHEJIANG UNIV
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