Metabonomics extraction method of pig gastrointestinal tract content
A technology of metabolomics and extraction method, which is applied in the field of metabolomics extraction of porcine gastrointestinal contents, and can solve the problems of inability to detect amino acid compounds at the same time, and the extraction procedure takes a long time.
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[0029] 1 content sample
[0030] Samples of cecal contents of 21-day-old piglets.
[0031] 2 experimental equipment
[0032] Analytical balance (Sartorius BS110S), Sartorius Company, Germany; micro-sampler, Thermo Company, USA; acidity meter (HANNA pH 211 type), HANNA Company, Italy; vortex oscillator (GL-8813 type), Qilin Bell Instruments, Haimen City, Jiangsu Province Manufacturing Co., Ltd.; low-temperature freezing large-capacity centrifuge (Anke DL-4000B type), Shanghai Anting Scientific Instrument Factory; ultra-low temperature refrigerator (SANYO Ultra low freezer), Japan SANYO company; Bruker Avance DRX-600 high-resolution nuclear magnetic resonance spectrometer, Germany Bruker Biospin Company.
[0033] 3 extraction methods:
[0034] Weigh 1 g of the thawed gastrointestinal tract content and adjust the pH value to 8.0 with 1M NaOH solution and 1M HCl solution, then weigh 0.1 g of the mixed content, add 0.1M phosphate buffer (100% heavy water, TSP in The standard co...
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