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A graphene quantum dot drug-loading system loaded with epirubicin and its preparation method

A technology of graphene quantum dots and epirubicin, applied in the field of biomedicine, can solve problems such as no research reports, and achieve the effects of high drug loading rate, mild experimental conditions, and improved bioavailability

Active Publication Date: 2017-05-17
南京美材科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention uses green graphene quantum dots as a drug carrier, loads the anti-tumor drug epirubicin to form an epirubicin graphene quantum dot drug-loading system, and analyzes the toxicity of cervical cancer Hela cells. There is no relevant research report

Method used

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  • A graphene quantum dot drug-loading system loaded with epirubicin and its preparation method
  • A graphene quantum dot drug-loading system loaded with epirubicin and its preparation method
  • A graphene quantum dot drug-loading system loaded with epirubicin and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Step 1 Self-made green graphene quantum dots.

[0045] Green graphene quantum dots are synthesized by hydrothermal method, see literature: Wang L, Wang YL, Xu T, et al, Nature communications, 2014, 5:5357.

[0046] First weigh 2 g of pyrene (purity>98%), measure 160 mL of concentrated nitric acid, mix them and put them into a round bottom flask. Under the condition of a constant temperature water bath, the mixture was heated to 80° C. and stirred by electric motor for 12 h to obtain a yellow solution, which was cooled to room temperature. Then measure 1 L of deionized water, dilute the mixture, put it into a filter press, and filter it with a 0.22um microporous membrane to remove the acid. Drying under vacuum gave 1,3,6-trinitropyrene.

[0047] Then weigh 3 g of the above-mentioned reactant 1,3,6-trinitropyrene, measure 50 mL of hydrazine hydrate solution and 10 mL of ammonia water respectively, mix them, put them into an ultrasonic cell pulverizer and sonicate for 1 ...

Embodiment 2

[0075] This embodiment is used as the comparative example of embodiment 1.

[0076] Step 1 Prepare commercially available blue graphene quantum dots

[0077] Step 2 Prepare epirubicin graphene quantum dot drug loading system

[0078] Prepare the epirubicin graphene quantum dot drug-loading system under the identical conditions of step 2 of Example 1;

[0079] Step 3 drug sustained release experiment

[0080] The same step 3 as in Example 1 was carried out for the sustained release experiment of the drug-loaded system.

[0081] Drawing of epirubicin standard curve: take a certain volume of PBS solution (pH=7.4) with a concentration of 0.05mg / mL EPI and dilute it into a series of solutions with known concentrations, and measure the concentration of these solutions at 485nm with a UV-visible spectrophotometer. The absorbance is plotted with absorbance to drug concentration to obtain the absorbance-concentration standard curve of epirubicin, and the equation of this standard cu...

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Abstract

The invention discloses an epirubicin loaded graphene quantum dot drug carrying system and a preparation method thereof. The preparation method includes the following steps: preparation of graphene quantum dots by a hydrothermal method; loading an anti-cancer drug with the graphene quantum dots; and performing a drug slow-release experiment, and performing a cytotoxicity test on the drug carrying system. The graphene quantum dots prepared by the hydrothermal method are used as a drug carrier for being loaded with epirubicin, greatly improve the drug loading rate, and show a quite good inhibitory effect on tumor HeLa cells. The preparation method of the drug carrying system is simple to operate, mild in reaction conditions and high in drug loading rate, and is expected to have a broad application prospect in the field of biological medicine.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a graphene quantum dot drug-loading system loaded with epirubicin, and the application of the system in anti-cervical cancer drugs. Background technique [0002] At present, the main problem in the clinical application of tumors is the separation of tumor diagnosis and treatment, which cannot fully reflect the clinical dynamic process of tumor patients during treatment, and cannot fully predict the therapeutic effect of tumors. Traditional chemotherapy drugs cannot effectively distinguish tumor cells from normal cells, resulting in toxicity and adverse reactions, and are prone to drug resistance. The use of drug-loaded systems can minimize the side effects of drugs, but optical properties are required to monitor the distribution of drugs in vivo and the progress of tumor treatment. Therefore, the design and application of a drug-loading system integrating chemotherapy and tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K47/04A61K31/704A61P35/00
Inventor 张小娟郝凌云田湘萍胡小红朱星群马俊杰
Owner 南京美材科技有限公司
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