Pasteurellosis and duck colibacilosis combined propolis inactivated vaccine and preparation method thereof

A duck pasteurellosis and colibacillosis technology, which is applied in the field of animal vaccine preparation, can solve the problems of economic loss, high mortality, and difficult radical cure in the duck industry, and reduce the incidence of duck pasteurellosis and duck colibacillosis The effect of occurrence, improvement of survival rate

Inactive Publication Date: 2015-11-04
山东华宏生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease can persist in the disease field, and it is easy to cause different batches of ducks to be infected. In addition, the disease is difficult to cure, causing serious economic losses to the duck industry.
[0007] Duck pasteurellosis and duck colibacillosis often appear in the form of mixed infection clinically, with high mortality, low feed conversion rate and high treatment cost, causing great economic losses to the duck industry

Method used

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  • Pasteurellosis and duck colibacilosis combined propolis inactivated vaccine and preparation method thereof
  • Pasteurellosis and duck colibacilosis combined propolis inactivated vaccine and preparation method thereof
  • Pasteurellosis and duck colibacilosis combined propolis inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) When in use, resuspend and dilute the above two inactivated antigen concentrates with physiological saline, and with reference to the colony counting results, the antigen concentrates of the Pasteurella duckis CAU0085 strain and the Escherichia coli CVCC1560 strain were diluted to The concentration is 2.0×10 9 CFU / ml.

[0057] (2) Mix the above two inactivated antigen concentrated diluents at a ratio of 1:1 to form a vaccine antigen solution.

[0058] (3) Take the vaccine antigen solution according to the amount of 60%, then add 7% astragalus polysaccharide and shake well, then take the propolis solution according to the amount of 33%, mix and stir for 30 minutes, make it fully mixed and emulsified, and form the vaccine emulsion ;

[0059] (4) Add the water for injection required for preparing the vaccine and stir for 5 minutes to prepare the double propolis inactivated vaccine for duck pasteurellosis and duck colibacillosis.

[0060] (5) Safety experiment of duc...

Embodiment 2

[0067] (1) When in use, resuspend and dilute the above two inactivated antigen concentrates with physiological saline, and with reference to the colony counting results, the antigen concentrates of the Pasteurella duckis CAU0085 strain and the Escherichia coli CVCC1560 strain were diluted to The concentration is 2.5×10 9 CFU / ml.

[0068] (2) Mix the above two inactivated antigen concentrated diluents at a ratio of 1:1 to form a vaccine antigen solution.

[0069] (3) Take the vaccine antigen solution according to the amount of 68%, then add 5% astragalus polysaccharide and shake it well, then take the propolis solution according to the amount of 27%, mix and stir for 30 minutes, make it fully mixed and emulsified, and form the vaccine emulsion ;

[0070] (4) Add the water for injection required for preparing the vaccine and stir for 5 minutes to prepare the double propolis inactivated vaccine for duck pasteurellosis and duck colibacillosis.

[0071] (5) Safety experiment of ...

Embodiment 3

[0078] (1) When in use, resuspend and dilute the above two inactivated antigen concentrates with physiological saline, and with reference to the colony counting results, the antigen concentrates of the Pasteurella duckis CAU0085 strain and the Escherichia coli CVCC1560 strain were diluted to The concentration is 3.0×10 9 CFU / ml.

[0079] (2) Mix the above two inactivated antigen concentrated diluents at a ratio of 1:1 to form a vaccine antigen solution.

[0080] (3) Measure the vaccine antigen solution according to the amount of 65%, then add 6% astragalus polysaccharide and shake well, then take the propolis solution according to the amount of 29%, mix and stir for 30 minutes, make it fully mixed and emulsified, and form the vaccine emulsion ;

[0081] (4) Add the water for injection required for preparing the vaccine and stir for 5 minutes to prepare the duck pasteurellosis and duck colibacillosis dual propolis inactivated vaccine.

[0082] (5) Safety experiment of duckli...

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Abstract

The invention belongs to the technical field of animal vaccine preparation, and particularly relates to a pasteurellosis and duck colibacilosis combined propolis inactivated vaccine and a preparation method thereof. Preparation of the combined propolis inactivated vaccine is completed by conducting propolis stock preparation, preparation of bacterial strains used for production, antigen liquid preparation, viable count, inactivation, ultrafiltration and concentration, vaccine security examination and protency examination on duck pasteurella CAU0085 bacterial strains and duck escherichia coli CVCC1560 bacterial strain antigens. The combined propolis inactivated vaccine is good in immune effect.

Description

technical field [0001] The invention belongs to the technical field of animal vaccine preparation, and in particular relates to a double propolis inactivated vaccine for duck pasteurellosis and duck colibacillosis and a preparation method thereof. Background technique [0002] Duck colibacillosis is a common disease of ducks, mainly affecting ducklings or ducklings aged 2 to 6 weeks. Onset duck farms generally have poor sanitary conditions, humidity, excessive stocking density, and poor ventilation. The onset is more common in late autumn and winter and spring, and can cause sepsis, so it is also called duck E. coli sepsis. [0003] Escherichia coli (E.Coli) is a Gram-negative bacillus with a size of 2 μm to 3 μm×0.6 μm and can grow well in most media. Grows on MacConkey agar to form red colonies. Most Escherichia coli are normal bacteria in human and animal intestines, and they are non-pathogenic bacteria to ducks. Only a few serotypes are pathogenic, which can cause duc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/102A61K39/108A61K39/39A61P31/04
CPCY02A50/30
Inventor 王永明王晓丽于海光张效伟李贝柴红任明明李士成
Owner 山东华宏生物工程有限公司
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