A system for extraction of cells from a sample of tissue

A technology of tissue samples and cells, which is applied in the system field of extracting cells from tissue samples, can solve problems such as the need for hydraulic circuits, standard performance deviations and influences of hydraulic circuits, achieve high sterility, reduce the risk of errors, and ensure safety Effect

Inactive Publication Date: 2015-11-04
SWISS STEM CELL FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this blood processor has a disadvantage that the fluid transfer via the pinch valve and squeeze system does not provide the high accuracy required for obtaining mesenchymal stem cells from adipose tissue
For example, accuracy may be affected by unforeseen factors including, for example, deviations from the standard performance of hydraulic circuits due to wear or degradation of gaskets or fluids
Also, the flow pattern caused by squeezing the bag is not exact
Another disadvantage is the need for a hydraulic circuit in the main unit

Method used

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  • A system for extraction of cells from a sample of tissue
  • A system for extraction of cells from a sample of tissue
  • A system for extraction of cells from a sample of tissue

Examples

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example

[0087] The following is an example of a protocol performed with the device and disposable kit of the present invention. The protocol is based on the fact that adipose tissue and hydrophilic fluid separate spontaneously in two phases without centrifugation.

[0088] The piston 19 of the separation chamber 11 within the device is used to absorb or expel a solution for washing the sample, separating the aspirated fat or extracting cells from the separated adipose tissue.

[0089] The disposable kit is filled with 150g of adipose tissue. In the first step, add 150ml of PBS / Ca from the first solution bag ++ Mg ++ Loading into chamber 11 completes the first washing step by gentle oscillation of the active capsule within the device. Next, the shaking is stopped and the capsule is allowed to stand vertically for a few minutes to allow the phases to separate and the lower aqueous phase is discarded by pushing the piston 19 . This procedure is repeated twice.

[0090] Next, the rem...

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Abstract

A system for extraction of cells from a sample of tissue comprises a main device (1) and a disposable kit (4) for use with said main device; said main device (1) is configured to host at least one disposable kit and to execute at least one preset program; said disposable kit (4) comprises a work chamber (11) for the sample of tissue and a plurality of solution bags (12), the disposable kit comprises also fluid transfer means (14, 19) operating between the work chamber and the solution bags (12) and controlled by the main device via suitable interface means; in a preferred embodiment the disposable kit (4) is mounted on a drum and contained in an active capsule carried by a rotary carousel; claims are also directed to the disposable kit for use in said system.

Description

technical field [0001] The present invention relates to a system for extracting cells from a tissue sample. The system can be used to extract stem cells, especially human mesenchymal stem cells (hMSC) from adipose tissue. Background technique [0002] Adult stem cells are of great potential interest in the fields of pharmacy and medicine. Adipose tissue is a promising source of stem cells suitable for general cell therapy. Extraction of mesenchymal stem cells from adipose tissue has great potential in combination with reconstructive surgery. Stem cells obtained from adipose tissue are currently used in breast reconstruction after partial mastectomy; further possible uses involve the treatment of a large number of diseases, including Parkinson's disease, Alzheimer's disease, bone marrow disease, cardio-cerebral crisis , burns, diabetes, rheumatoid arthritis and other diseases. Adipose tissue is readily available, accounting for at least 10% of an individual's body weight;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/33C12M1/00A61M1/02B01L3/00
CPCC12M45/09A61M2202/0437A61M2202/08B01L3/502C12M23/28C12M23/34C12M23/40C12M45/02C12M47/04C12M45/05B01L3/567B01L7/00B01L2400/0403C12N5/0667
Inventor 毛罗·戈拉詹尼·索尔达蒂
Owner SWISS STEM CELL FOUND
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