A system for extraction of cells from a sample of tissue
A technology of tissue samples and cells, which is applied in the system field of extracting cells from tissue samples, can solve problems such as the need for hydraulic circuits, standard performance deviations and influences of hydraulic circuits, achieve high sterility, reduce the risk of errors, and ensure safety Effect
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[0087] The following is an example of a protocol performed with the device and disposable kit of the present invention. The protocol is based on the fact that adipose tissue and hydrophilic fluid separate spontaneously in two phases without centrifugation.
[0088] The piston 19 of the separation chamber 11 within the device is used to absorb or expel a solution for washing the sample, separating the aspirated fat or extracting cells from the separated adipose tissue.
[0089] The disposable kit is filled with 150g of adipose tissue. In the first step, add 150ml of PBS / Ca from the first solution bag ++ Mg ++ Loading into chamber 11 completes the first washing step by gentle oscillation of the active capsule within the device. Next, the shaking is stopped and the capsule is allowed to stand vertically for a few minutes to allow the phases to separate and the lower aqueous phase is discarded by pushing the piston 19 . This procedure is repeated twice.
[0090] Next, the rem...
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