Preparing method of sea anemone crude extract and anti-tumor application of sea anemone crude extract

A technology of crude extract and sea anemone, which is applied in the direction of antineoplastic drugs, drug combinations, medical raw materials derived from cnidaria, etc., can solve problems such as toxic and side effects, and achieve good biological activity and strong antitumor activity in vitro

Inactive Publication Date: 2015-11-11
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing clinical anti-lung cancer drugs often have relatively large toxic and side effects while exerting anti-cancer effects. Therefore, the research and development of drugs with high activity, definite curative effect, and low toxic and side effects has become a key issue in the research of anti-lung cancer drugs.

Method used

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  • Preparing method of sea anemone crude extract and anti-tumor application of sea anemone crude extract
  • Preparing method of sea anemone crude extract and anti-tumor application of sea anemone crude extract
  • Preparing method of sea anemone crude extract and anti-tumor application of sea anemone crude extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of embodiment 1 sea anemone crude extract

[0023] 1) Wash the reared live sea anemone in pure water to remove surface impurities, put the washed sea anemone in a beaker, add a certain amount of pure water according to the material ratio of 1:1.5, and place it at -60°C Freeze overnight at ultra-low temperature, take it out the next day and thaw naturally at 20°C, after it is completely dissolved, put it in the freezer to freeze, and cycle the freezing and thawing steps 3 times;

[0024] 2) The sea anemone wrapped in gauze in step 1) is completely thawed at 20°C, and then coarsely filtered, and the impurities in the sea anemone body are discarded;

[0025] 3) Take the filtrate, centrifuge the filtrate at 4°C and 12000r / min for 10min, collect the supernatant to obtain the sea anemone crude venom;

[0026] 4) Add the sea anemone crude venom in step 3) to 30%, 60%, and 90% acetone pre-cooled at -20°C for fractional precipitation, and stir with a glass rod t...

Embodiment 2

[0027] The preparation of embodiment 2 sea anemone crude extract

[0028] 1) Wash the reared live sea anemone in pure water to remove surface impurities, put the washed sea anemone in a beaker, add a certain amount of pure water according to the material ratio of 1:2, and place it at -60°C Freeze overnight at ultra-low temperature, take it out the next day and thaw naturally at 22°C. After it is completely dissolved, put it in the freezer to freeze, and cycle the freezing and thawing steps twice;

[0029] 2) The sea anemone wrapped in gauze in step 1) is completely thawed at 28°C, and then coarsely filtered, and the impurities in the sea anemone body are discarded;

[0030] 3) Take the filtrate, centrifuge the filtrate at 6°C and 14000r / min for 15min, collect the supernatant to obtain the sea anemone crude venom;

[0031] 4) Add the sea anemone crude venom in step 3) to 30%, 60%, and 90% acetone pre-cooled at -20°C for fractional precipitation, and stir with a glass rod to ra...

Embodiment 3

[0032] The preparation of embodiment 3 sea anemone crude extract

[0033] 1) Wash the reared live sea anemone in pure water to remove surface impurities, put the washed sea anemone in a beaker, add a certain amount of pure water according to the material ratio of 1:1.8, and place it at -60°C Freeze overnight at ultra-low temperature, take it out the next day and thaw naturally at 20°C, after it is completely dissolved, put it in the freezer to freeze, and cycle the freezing and thawing steps 3 times;

[0034] 2) The sea anemone wrapped in gauze in step 1) is completely thawed at 25° C., and then coarsely filtered, and the impurities in the sea anemone body are discarded;

[0035] 3) Take the filtrate, centrifuge the filtrate at 5°C and 13000r / min for 12min, collect the supernatant to obtain the sea anemone crude venom;

[0036] 4) Add the sea anemone crude venom in step 3) to 30%, 60%, and 90% acetone pre-cooled at -20°C for fractional precipitation, and stir with a glass rod...

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Abstract

The invention relates to a preparing method of sea anemone crude extract. The preparing method includes the steps of 1, washing bred live sea anemones in pure water, placing the washed sea anemones in a beaker, adding pure water into the beaker according to a sea anemone to pure water ratio being 1:1.5 to 1:2, and performing freeze thawing twice to three times at -60 DEG C; 2, moderately unfreezing the sea anemones obtained in the step 1, and performing coarse filtration; 3, centrifuging filtrate obtained in the step 2 at 4 DEG C to 6 DEG C at the speed of 12000-14000 r / min for 10 to 15 min, and collecting supernate to obtain sea anemone crude venom; 4, subjecting the sea anemone crude venom obtained in the step 3 to fractional precipitation with acetone, centrifuging precipitate obtained in each step for 15min, collecting final precipitate, and freeze-drying the final precipitate to obtain sea anemone crude toxin. The invention further provides application of the sea anemone crude toxin in the preparation of anti-lung cancer medicaments.

Description

technical field [0001] The invention relates to the field of deep processing of marine products, in particular to a preparation method of crude sea anemone extract and its antitumor application. Background technique [0002] Sea anemone, also known as sea chrysanthemum, is a marine coelenterate belonging to the phylum Coelenterata, class Anthozoa, and order actiniaria. It is a relatively primitive animal in the ocean. There are 37 species of sea anemones in 6 families, widely distributed in tropical and temperate tropical seas, mainly fixed on rocks or in sand in the sea. Sea anemone has the effects of nourishing yin and strengthening yang, astringent and antidiarrheal, dampness and insecticide. The research on the chemical composition of sea anemones began in the 1970s. The tentacles and bodies of sea anemones contain numerous stinging cells, among which are organelles that store venom—nematocysts. When sea anemones encounter physical or chemical stimuli, The barbed wire ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/614A61P35/00
Inventor 杨最素张亚茹丁国芳黄芳芳赵玉勤余方苗
Owner ZHEJIANG OCEAN UNIV
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