Cordyceps militaris liquid culture medium and culture method of cordyceps militaris

A technology of liquid culture medium and Cordyceps militaris, applied in the biological field, can solve the problems of high production cost, low product recovery rate, complex synthesis process, etc., and achieve the effects of short production cycle, convenient extraction and increased yield

Inactive Publication Date: 2015-11-11
中山鼎晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The content of cordycepin in Cordyceps militaris is extremely low. Although cordycepin can be chemically synthesized, the synthesis process is complicated, the product recovery rate is low, and the production cost is high. Therefore, how to obtain cordycepin through fermentation of Cordyceps militaris is the most researched at present, but How to optimize the culture conditions of Cordyceps militaris, improve the growth of Cordyceps militaris and the content of cordycepin in it has always been a concern.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of Cordyceps militaris liquid culture medium, described culture medium comprises and is made up of each component of following content:

[0035] KH2PO41g / L,

[0036] MgSO41g / L,

[0037] Peptone 5g / L,

[0038] Potato 200g / L,

[0039] D-fructose 8g / L,

[0040] Agar 20g / L,

[0041] Arginine 10g / L,

[0042] Glutamic acid 8g / L,

[0043] Silkworm chrysalis powder 30g / L.

[0044] The pH of the medium is 5.

[0045] Adopt the above-mentioned Cordyceps militaris liquid culture medium to carry out the method for Cordyceps militaris cultivation, comprise the following steps:

[0046] (1) Shake flask culture: according to KH2PO41g / L, MgSO41g / L, peptone 5g / L, potato 200g / L, D-fructose 8g / L, agar 20g / L, arginine 10g / L, glutamic acid 8g / L L, silkworm chrysalis powder 30g / L basal culture medium formula is carried out formulary base preparation, high-pressure steam sterilization 10min, after cooling at normal temperature, be packed in the jar of 500ml, press the volume r...

Embodiment 2

[0050] A kind of Cordyceps militaris liquid culture medium, described culture medium comprises and is made up of each component of following content:

[0051] KH2PO43g / L,

[0052] MgSO43g / L,

[0053] Beef extract 20g / L,

[0054] Potato 500g / L,

[0055] D-fructose 15g / L,

[0056] Agar 50g / L,

[0057] Lysine 25g / L,

[0058] Aspartic acid 20g / L,

[0059] Silkworm chrysalis powder 50g / L.

[0060] The pH of the medium is 8.

[0061] Adopt the above-mentioned Cordyceps militaris liquid culture medium to carry out the method for Cordyceps militaris cultivation, comprise the following steps:

[0062] (1) Shake flask culture: according to KH2PO43g / L, MgSO43g / L, beef extract 20g / L, potato 500g / L, D-fructose 15g / L, agar 50g / L, lysine 25g / L, aspartic acid 20g / L, silkworm chrysalis powder 50g / L basal culture medium formula carries out the preparation of supporting medium, autoclave steam sterilization 30min, after cooling at normal temperature, be packed in the jar of 500ml, press...

Embodiment 3

[0066] A kind of Cordyceps militaris liquid culture medium, described culture medium comprises and is made up of each component of following content:

[0067] KH2PO42g / L,

[0068] MgSO42g / L,

[0069] Peptone 15g / L,

[0070] Potato 350g / L,

[0071] D-fructose 12g / L,

[0072] Agar 35g / L,

[0073] Histidine 18g / L,

[0074] Glutamic acid 15g / L,

[0075] Silkworm chrysalis powder 45g / L.

[0076] The pH of the medium is 7.

[0077] Adopt the above-mentioned Cordyceps militaris liquid culture medium to carry out the method for Cordyceps militaris cultivation, comprise the following steps:

[0078] (1) Shake flask culture: according to KH2PO42g / L, MgSO42g / L, peptone 15g / L, potato 350g / L, D-fructose 12g / L, agar 35g / L, histidine 18g / L, glutamic acid 15g / L L, silkworm chrysalis powder 45g / L basal culture medium formula is carried out formulating culture base preparation, autoclave steam sterilization 20min, after cooling at normal temperature, be packed in the jar of 500ml, pres...

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Abstract

The invention relates to a cordyceps militaris liquid culture medium which comprises the following components: 1-3 g / L of KH2PO4, 1-3 g / L of MgSO4, 5-20 g / L of a nitrogen source, 200-500 g / L of potatoes, 8-15 g / L of D-fructose, 20-50 g / L of agar, 10-25 g / L of basic amino acid, 8-20 g / L of acidic amino acid, and 30-50 g / L of silkworm chrysalis meal. The liquid culture medium provided by the invention uses D-fructose as a carbon source; basic amino acid and acidic amino acid are added to the culture medium; the yield of the cordyceps militaris cultured by using the liquid culture medium is remarkably improved, and the production of cordycepin in the cordyceps militaris is also improved; the production cycle is short; the follow-up extraction and separation of cordycepin are convenient; the cordyceps militaris liquid culture medium is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a liquid culture medium of Cordyceps militaris and a cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the phylum Ascomycota, Sordariomycetes, Hypocreales, Cordycipitaceae, Cordyceps, and is the model species of the genus Cordyceps. Wild Cordyceps militaris mainly parasitizes on the pupae of Lepidoptera insects in the topsoil of coniferous forests, broad-leaved forests or mixed forests, and is also found to parasitize on pupae, adults or larvae of insects such as Coleoptera and Diptera. Cordyceps militaris contains a variety of biologically active substances, including cordycepin, adenosine and other nucleoside substances, as well as cordyceps polysaccharides, SOD (superoxide dismutase) and amino acids, and the most important active ingredient is cordycepin. It shows that co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40C12R1/645
Inventor 郭狄
Owner 中山鼎晟生物科技有限公司
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