Application of dracocephalum moldavica total flavonoids to preparation of drug for preventing and treating AD (Alzheimer's disease)

A technology for Alzheimer's disease and total flavonoids, applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., can solve problems such as learning and memory ability of total flavonoids of vanilla japonica that have not yet been found, and achieve shortened escape latency, neuron protection, The effect of reducing MDA content

Inactive Publication Date: 2015-11-25
胡艳丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no report has been found on the application of total flavonoids of Xiangqinglan in the prevention and treatment of learning and memory impairment caused by Alzheimer's disease

Method used

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  • Application of dracocephalum moldavica total flavonoids to preparation of drug for preventing and treating AD (Alzheimer's disease)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Effects of total flavonoids of sweet green orchid on learning and memory of dementia mice

[0013] 1. Experimental materials

[0014] (1) Experimental drug: total flavonoids of Xiangqinglan (made by the School of Pharmacy, Shihezi University, with a purity of 82.3%).

[0015] (2) Animals: 50 Kunming mice, male, weighing (23±2) g, provided by the Experimental Animal Center of Shihezi University.

[0016] (3) Reagents: Sodium nitrite and D-galactose are commercially available analytically pure;

[0017] (4) Instrument: Morris water maze (developed by Institute of Materia Medica, Chinese Academy of Medical Sciences);

[0018] 2. Experimental method

[0019] (1) Establishment of dementia mouse model

[0020] 50 Kunming mice were randomly divided into 5 groups, 10 in each group, which were blank control group, model group, low-dose group of total flavonoids of Xiangqinglan (25mg / kg), middle-dose group of total flavonoids of Xiangqinglan (50mg / kg). kg), the hi...

Embodiment 2

[0033] Example 2: The effect of total flavonoids of Cymbidium on the level of oxidative stress in the brain tissue of dementia mice

[0034] 1. Experimental materials

[0035] (1) Reagents: malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) kits were purchased from Nanjing Jiancheng Bioengineering Institute;

[0036] (2) Instruments: microplate reader (Thermo3001, American Thermo Company); TGL-16G cryogenic refrigerated centrifuge (Shanghai Anting Scientific Instrument Factory);

[0037] 2. Experimental method

[0038] After the water maze experiment, the animals were sacrificed, the whole brain was removed and the blood was washed with ice-cold saline, and the cerebral cortex was separated under a low temperature environment. After weighing, 9 times the volume of saline was added to make a 10% homogenate, and centrifuged at 3500r / min for 10min. The supernatant was taken to determine the contents of malondialdehyde, superoxide dismutase and...

Embodiment 3

[0052] Example 3: Effects of total flavonoids of Xiangqinglan on the level of brain tissue inflammation in dementia mice

[0053] 1. Experimental materials

[0054] (1) Reagents: Interleukin 1β (1L-1β), tumor necrosis factor α (TNF-α) ELISA kit (Wuhan Boster Bioengineering Co., Ltd.). Inducible Nitric Oxide (NOS2) Detection Kit (Wuhan Yousheng Trading Co., Ltd.);

[0055] (2) Instruments: microplate reader (Thermo3001, American Thermo Company); TGL-16G cryogenic refrigerated centrifuge (Shanghai Anting Scientific Instrument Factory);

[0056] 2. Experimental method

[0057] After the water maze experiment, the animals were sacrificed, the whole brain was removed and the blood was washed with ice-cold saline, and the cerebral cortex was separated under a low temperature environment. After weighing, 9 times the volume of saline was added to make a 10% homogenate, and centrifuged at 3500r / min for 10min. The supernatant was taken to determine the contents of interleukin-1β, tum...

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Abstract

The invention discloses an application of dracocephalum moldavica total flavonoids to preparation of a drug for preventing and treating the AD (Alzheimer's disease). An AD mouse model is established through hypodermic injection of sodium nitrite/D-galactose, the learning and memory capacity and biochemical indexes are scientifically assessed through mouse positioned navigation tests and space exploration tests, it proves that the dracocephalum moldavica total flavonoids can improve the space learning and memory capacity of AD model mice, dose dependence is formed, escape incubation of the AD mice is shortened, and platform crossing times in the space exploration tests are significantly increased; meanwhile, the content of MDA in cortical tissue in the AD mice can be significantly decreased, the content of SOD can be significantly increased, and the oxidative stress level in brain tissue can be reduced; also, through reduction of expression of pro-inflammatory factors IL-1beta and TNF-alpha, inflammatory injuries to the brain tissue can be relieved, the neuron protection role can be played, and the learning and memory capacity of the AD mice can be improved finally. Thus, a reference basis can be provided for clinic application of the dracocephalum moldavica total flavonoids in the future.

Description

technical field [0001] The invention relates to the application of the total flavonoids of the active ingredients of the medicine, and in particular to the application of the total flavonoids of the saffron in the preparation of drugs for preventing and treating Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD), also known as senile dementia, is a common neurodegenerative disease. It is a common disease in the elderly after heart disease, tumor and cerebrovascular disease. , and is the most common cause of dementia in the elderly. Clinically, it is characterized by memory loss and cognitive dysfunction, and the condition is progressively aggravated, and the ability to live independently is lost within a few years, and death is often caused by concurrent infection in about 10 years. Epidemiological surveys show that the incidence of AD over the age of 65 is 5-10%, and the incidence will double every 5 years thereafter, and the incidence of AD over t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P25/28
Inventor 胡艳丽翟薇张璐
Owner 胡艳丽
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