A kind of production identification method of Lactobacillus plantarum jla-9, and the bacteriocin produced by it, and bacteriocin
A technology of Lactobacillus plantarum and a production method, applied in the field of novel lactobacillus bacteriocin, can solve problems such as less application, achieve the effects of good application prospect, high human health and food safety, and prolonging shelf life
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] Example 1 Screening of anti-bacillus lactic acid bacteria
[0033] 1. Isolation and purification of lactic acid bacteria: 25 grams of sauerkraut samples collected (food sauerkraut made by traditional fermentation methods in Jilin Province, China) were put into 225ml sterile saline for 10-fold gradient dilution, and 10 -4 , 10 -5 10 -6 0.1 ml of each of the three concentrations was spread on the MRS solid medium plate containing 2% calcium carbonate (2 g calcium carbonate in 100 ml medium). Randomly pick single colonies with calcium-dissolved circles, and further separate and purify the obtained single colonies by streaking on the MRS solid medium plate containing calcium carbonate, and select those with obvious calcium-dissolved circles (that is, transparent circles can be seen with the naked eye) ) single colony, further pass the catalase test and Gram staining, select a single colony that is negative in the catalase test and positive in Gram staining, and further st...
Embodiment 2
[0052] Embodiment 2 Identification of novel lactic acid bacteria bacteriocin
[0053] 1. Fermentation of Lactobacillus plantarum to produce bacteriocins
[0054] (1) Preparation of Lactobacillus plantarum JLA-9 seed liquid: inoculate Lactobacillus plantarum JLA-9 on the MRS agar medium on the slant of a test tube, culture at 37°C for 24 hours, and activate the strain; then the activated strain Seeds were inoculated in MRS liquid medium, cultured statically at 37°C for 16-20 hours, and reached the logarithmic growth phase, and the concentration was 10 7 ~10 8 cfu / ml seed solution;
[0055] (2) Fermentation of Lactobacillus plantarum JLA-9 producing bacteriocin: inoculate the seed solution of Lactobacillus plantarum JLA-9 in MRS fermentation medium at a concentration of 1%, and cultivate it under static conditions at 37°C for 36 hours to obtain a bacteriocin fermentation broth .
[0056] 2. Isolation of bacteriocin produced by Lactobacillus plantarum (Lactobacillus plantarum...
Embodiment 3
[0063] Example 3 The antibacterial activity of novel lactic acid bacteria bacteriocin
[0064] The purified bacteriocins were selected from Gram-positive bacteria and Gram-negative bacteria described in Table 4 (respectively from CMCC, ATCC, CICC, etc.) as indicator strains, and their antibacterial activity was determined. The results showed that the bacteriocin produced by Lactobacillus plantarum JLA-9 had good antibacterial effect on Gram-positive bacteria and Gram-negative bacteria, especially had a broad-spectrum antibacterial effect on spore-like bacteria.
[0065] Table 4 Antibacterial spectrum of bacteriocin produced by Lactobacillus plantarum JLA-9
[0066]
[0067]
[0068]
[0069]
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More