High-producing 3-hydroxypropionaldehyde strain and application thereof to prevention and treatment of digestive tract infection of mammals
A technology for high production of hydroxypropionaldehyde and digestive tract infection, applied in the direction of microorganism-based methods, anti-infective drugs, biochemical equipment and methods, etc., can solve problems such as liver and kidney function damage, bacterial flora imbalance, allergic reactions, etc., and achieve growth The effect of cycle shortening, production cost reduction, and broad-spectrum antibacterial effect
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[0035] The method for the production of 3-hydroxypropanal of the probiotic high-yield bacterial strain among the present invention, carries out according to the following steps:
[0036] 1. Preparation medium: peptone 10g, beef extract 10g, glucose 20g, yeast extract 5g, Tween 801ml, dipotassium hydrogen phosphate 2g, triammonium citrate 2g, sodium acetate 5g, magnesium sulfate 0.5g, manganese sulfate 0.2g , L-cysteine 0.5g, sodium thioglycolate 0.05g, VB120.005g, glycerol 3.68g add water to 1L, adjust the pH value to 6.8;
[0037] 2. Insert Lactobacillus reuteri RS01 of the present invention into the culture medium prepared in step 1, and perform anaerobic fermentation at 37°C for 5h;
[0038] 3. Separation: The plate and frame separation is used for the first separation, and the collected bacteria are fast and not easy to cause the death of the bacteria; then the separated bacteria are inserted into a new medium, and the dissolved oxygen is 0 at 37 ° C. , cultivated for 4...
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