Method for preparing extract with alpha-glucosidase restraining effect

A technology of glucosidase and inhibition, which is applied in the field of preparation of hawthorn leaf extract, can solve the problems of extraction of ineffective components, low inhibition of enzyme activity, inability to be used as health food, etc., and achieves a simple and feasible preparation method and a wide range of sources Effect

Inactive Publication Date: 2015-12-02
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The currently commercially available hypoglycemic drugs with α-glucosidase inhibitory effect are mainly chemically synthesized drugs, such as acarbose, O-4,6-dideoxy-4[[(1S,4R,5S,6S)4 ,5,6-Trihydroxy-3-(hydroxymethyl)-2-cyclohexene]amino]-(-D-glucopyranosyl(1→4)-O-)-D-glucopyranose Base (1→4)-D-glucopyranose, non-natural, only used as medicine, can not be used as health food, etc.
[0006] However, natural plant products are mostly directly used as brewed drinks such as tea, without extraction of specific functional components, and low enzyme activity inhibition

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation method of the hawthorn leaf extract with high α-glucosidase inhibitory effect of hawthorn leaves is as follows:

[0039] Step 1. Hawthorn leaves are dried and crushed to 100 meshes to prepare hawthorn leaf powder

[0040] Step 2, using the ultrasonic-assisted method, according to hawthorn leaf powder: ethanol = 1:35 (w / v, mass volume ratio, the unit of this embodiment is selected as grams: milliliter, hawthorn leaf powder: ethanol = 1:35 (w / v ) is 1 gram of hawthorn leaf powder: 35 milliliters of ethanol) ratio to extract the crude solution, the single extraction time is 40 minutes, the ultrasonic power is 480 watts, and the ethanol concentration is 70%; then the extracted crude solution is obtained by ultrafiltration to obtain the filtrate; repeated co-extraction and Ultrafiltration 3 times (the filter residue was extracted twice more according to the above process), and the filtrate obtained by 3 repeated extractions and ultrafiltration was combined in...

Embodiment 2

[0046] The preparation method of the hawthorn leaf extract with high α-glucosidase inhibitory effect of hawthorn leaves is as follows:

[0047] Step 1, hawthorn leaves are dried and pulverized into 80 meshes to prepare hawthorn leaf powder;

[0048]Step 2, using the ultrasonic-assisted method, extract the crude liquid according to the ratio of hawthorn leaf powder: ethanol=1:20 (w / v), the single extraction time is 50 minutes, the ultrasonic power is 400 watts, and the ethanol concentration is 85%; liquid through ultrafiltration to obtain the filtrate; repeated co-extraction and ultrafiltration for 2 times (repeated extraction and ultrafiltration of the filter residue according to the above-mentioned process for 1 time), the filtrate obtained by repeated extraction and ultrafiltration for 2 times was combined into hawthorn leaf Extraction solution;

[0049] Step 3, the hawthorn leaf extract is concentrated under reduced pressure (vacuum distillation method) to remove ethanol, ...

Embodiment 3

[0054] The preparation method of the hawthorn leaf extract with high α-glucosidase inhibitory effect of hawthorn leaves is as follows:

[0055] Step 1, hawthorn leaves are dried and pulverized into 120 meshes to prepare hawthorn leaf powder;

[0056] Step 2, using the ultrasonic-assisted method, extract the crude liquid according to the ratio of hawthorn leaf powder: ethanol=1:40 (w / v), the single extraction time is 50 minutes, the ultrasonic power is 600 watts, and the ethanol concentration is 60%; the extracted crude liquid The filtrate was obtained by ultrafiltration; after repeated co-extraction and ultrafiltration 3 times (repeated extraction and ultrafiltration of the filter residue according to the above process 2 times), the filtrate obtained by repeated extraction and ultrafiltration 3 times was combined into Hawthorn Leaf Extract liquid;

[0057] Step 3, the hawthorn leaf extract is concentrated under reduced pressure (vacuum distillation method) to remove ethanol, ...

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Abstract

The invention discloses a preparation method for Chinese hawthorn leaf extract with the alpha-glucosidase restraining effect. The method includes the following steps of preparing Chinese hawthorn leaf powder, preparing a Chinese hawthorn leaf extracting solution through an ultrasonic assistance method, obtaining a concentrated extracting solution, obtaining a water-insoluble object, conducting chromatography on the water-insoluble object through polyacrylamide, obtaining effluent with a methanol solution as eluant, and conducting pressure-reduction concentrating and drying to obtain the target extract. The separation, extraction and preparation method is easy and feasible, and has the industrialized preparation potential; the enzyme activity restraining rate is higher than that of existing acarbose sold on the market; raw materials are wide in source, low in price and easy to obtain, and comprehensive utilization of by-products is achieved.

Description

technical field [0001] The invention relates to a preparation method of an extract, more specifically, to a preparation method of a hawthorn leaf extract. Background technique [0002] Diabetes mellitus (DM) is a disease of endocrine and metabolic disorders caused by insufficient insulin content in human blood. With the improvement of living standards, the number of diabetic patients at home and abroad is increasing. According to WHO statistics, by 2030, there will be as many as 366 million diabetic patients. Therefore, reducing postprandial blood sugar is an important measure to prevent diabetes and reduce its complications. [0003] α-Glucosidase Inhibitor (α-Glucosidase Inhibitor) inhibits the activity of α-glucosidase in the small intestine through competitive inhibition, reduces the decomposition rate of carbohydrates, and delays the absorption of glucose by the human body, thereby effectively reducing blood sugar concentration after meals Peak. Because α-glucosidase ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/734A61P3/10G01N21/31
Inventor 王锋顾丰颖梁俊弓威贺凡宁吉英高萍萍
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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