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Novel pterocarpan type flavonoid and applications

A technology of flavonoids and pterostane, which is applied in the field of medicine, can solve the problems of few research reports on yuqian, and achieve the effects of simple and fast preparation method, high yield and simple process

Active Publication Date: 2018-12-07
EFFEPHARM (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor consulted a large number of documents and found that there are relatively few research reports on the hypoglycemic activity of Yuqian

Method used

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  • Novel pterocarpan type flavonoid and applications
  • Novel pterocarpan type flavonoid and applications
  • Novel pterocarpan type flavonoid and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of embodiment 1 pterostane type flavonoids

[0028] (1), cut or pulverize the dry elm money (12.0 kg) into small pieces (0.5 cm), soak it with 88% ethanol for 10 hours at room temperature, then carry out heat reflux extraction for 3 times, each time for 3 hours, and filter , the combined filtrates were dried at 48°C under vacuum until there was no alcohol smell, to obtain the total extract of Yuqian (0.95 kg);

[0029] (2), uniformly suspending the total extract of Ulmus chinensis in distilled water (total extract of Ulmus chinensis: distilled water=1:2, V / V), let it stand for 19 hours, and filter to obtain the supernatant;

[0030] (3), the supernatant is extracted successively with sherwood oil, ethyl acetate, n-butanol, and each extract is concentrated under reduced pressure to obtain sherwood oil extract (92.5 grams), ethyl acetate extract (350.0 grams), n-butanol Butanol extract (406.8 grams); 3 kinds of thick extracts carry out α-glucosidas...

Embodiment 2

[0041] The α-glucosidase inhibitory activity assay of embodiment 2 compound I

[0042] (1) Experimental method

[0043] According to the method of Pierre et al. [1-2] , using p-nitrophenyl-β-D-glucopyranoside (PNPG) as the substrate to measure the activity of α-glucosidase.

[0044] Add 10 μL of α-glucosidase with a concentration of 1 U / mL (prepared in 0.1mol / L pH=6.8 phosphate buffer, the same below) to 100 μL buffer (0.1mol / L pH=6.8 phosphate buffer, the same below), at 37 Incubate at ℃ for 10 min, then add 90 μL p-nitrophenyl-β-D-glucopyranoside (PNPG) at a concentration of 0.01 mol / L, incubate at 37 °C for 10 min, and measure the absorbance at a wavelength of 405 nm with a microplate reader (A 酶 ).

[0045] Compound I was dissolved in DMSO to make a mass concentration of 0.625 g / L. Add 10 μL of α-glucosidase at a concentration of 1 U / mL to the sample, add 50 μL of buffer solution and incubate at 37°C for 10 minutes, then add 90 μL of 0.01mol / LPNPG at a concentration of...

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Abstract

The invention discloses a pterocarpan type flavonoid separated from elm fruits and shown as formula I, and also discloses a preparation method of the pterocarpan type flavonoid and applications of thepterocarpan type flavonoid for preparing hypoglycemic drugs. The pterocarpan type flavonoid has alpha-glucosidase inhibitory activity, so that the pterocarpan type flavonoid can be used for preparingthe hypoglycemic drugs; and the preparation method is convenient and fast, simple in technology, high in yield and good in purity, so that the preparation method has potential for industrial preparation. The pterocarpan type flavonoid is shown as the formula I.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a new pterostane type flavonoid compound and its application in the preparation of hypoglycemic drugs. Background technique [0002] Elm money is the samara of Ulmaceae plant Ulmus. Among the folks, elm money, as a common wild vegetable, is rich in nutrients and is deeply loved by people. According to ancient books, elm money has the functions of dredging stranguria and eliminating dampness and heat, and it is mainly used to treat excessive leucorrhea in women and malnutrition in children; external use can treat persistent diseases such as sores and ringworm. Modern research has found that in addition to rich nutritional value, elm money also has the functions of promoting children's growth and development, lowering human cholesterol, and losing weight. The inventor consulted a large number of documents and found that there are relatively few research reports on the hypoglycemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/14A61P3/10
CPCA61P3/10C07D493/14
Inventor 马勤阁魏荣锐杨明钟国跃
Owner EFFEPHARM (SHANGHAI) CO LTD
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