Organic fertilizer prepared from leaf of Sapium discolor and leaf of white poplar
A technology of organic fertilizer and tallow tree leaves, which is applied in the field of fertilizer and organic fertilizer, and can solve the problems of no prevention and control of rapeseed sclerotinia
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Embodiment 1
[0048] The preparation method of organic fertilizer is as follows:
[0049] (1) strain expansion culture: get white rot fungus, Aspergillus niger, inoculate in liquid PDA culture medium, shake table culture and carry out expansion, make containing bacteria liquid;
[0050] (2) Preparation of solid medium: Weigh 1 part of tallow tree leaf and 1 part of poplar leaf according to the mass, crush them, put them in an oven at 70°C to dry, add 300 g of water, 1 g of glucose, and 0.5 g of urea per 100 g of basic raw materials Add water, glucose and urea, mix well, as a solid medium;
[0051] (3) Solid fermentation: inoculate at the ratio of adding 10 mL of the bacterium-containing liquid per 100 g of basic raw materials, and cultivate for 6 days at 28-35° C.;
[0052] (4) Sterilization and drying: take the material obtained in step (3), sterilize it under high pressure, put it in an oven at 100°C and dry it to obtain the final product.
Embodiment 2
[0054] The preparation method of organic fertilizer is as follows:
[0055] (1) strain expansion culture: get white rot fungus, Aspergillus niger, inoculate in liquid PDA culture medium, shake table culture and carry out expansion, make containing bacteria liquid;
[0056] (2) Preparation of solid medium: Weigh 1 part of tallow tree leaves and 1 part of poplar leaves by weight, crush them, put them in an oven at 90°C to dry, add 400 g of water, 2 g of glucose, and 0.5 g of urea per 100 g of basic raw materials Add water, glucose and urea, mix well, as a solid medium;
[0057] (3) Solid fermentation: inoculate according to the ratio of adding 30 mL of the bacteria-containing solution per 100 g of basic raw materials, and cultivate for 10 days at 28-35 ° C;
[0058] (4) Sterilization and drying: take the material obtained in step (3), sterilize it under high pressure, put it in an oven at 100°C and dry it to obtain the final product.
Embodiment 3
[0060] The preparation method of organic fertilizer is as follows:
[0061] (1) strain expansion culture: get white rot fungus, Aspergillus niger, inoculate in liquid PDA culture medium, shake table culture and carry out expansion, make containing bacteria liquid;
[0062] (2) Preparation of solid medium: Weigh 1 part of Chinese tallow tree leaf and 1 part of poplar leaf according to the mass, crush them, place them in an oven at 80°C to dry, add 350 g of water, 1.5 g of glucose, and 0.5 g of urea per 100 g of basic raw materials. Proportionally add water, glucose and urea, mix well, as a solid medium;
[0063] (3) Solid fermentation: inoculate at the ratio of adding 20 mL of the bacterium-containing liquid per 100 g of basic raw materials, and cultivate for 8 days at 28-35° C.;
[0064] (4) Sterilization and drying: take the material obtained in step (3), sterilize it under high pressure, put it in an oven at 100°C and dry it to obtain the final product.
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