Process utilizing enzymatic removing impurity to prepare tussah pupa skin chitosan

A technology of tussah pupa and chitosan, applied in the biological field, can solve the problems of uneven product structure, a large amount of waste liquid waste water, chitin depolymerization, etc., and achieves easy collection and treatment, less waste water and waste liquid, and short production cycle. Effect

Inactive Publication Date: 2015-12-02
JILIN SERICULTURE SCI RES INST
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

This method requires a large amount of acid-base solution, and it is easy to produce a large amount of waste liquid and waste water after production, causing environmental pollution, ecological dama

Method used

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Embodiment Construction

[0019] The present invention will be further described below in combination with specific embodiments.

[0020] A kind of process of preparing tussah silkworm chrysalis skin chitosan utilizing enzymatic method to remove impurities, it comprises: utilize 10-15% citric acid as inorganic salt removal reagent, utilize alkaline protease to hydrolyze the protein in the tussah silkworm chrysalis skin again, The desalted and deproteinized product was washed with 1% H 2 o 2 For decolorization, the acetyl group of chitin is removed by concentrated alkali thermochemical method, and after purification, chitosan that meets the standard is finally obtained. The specific process steps are as follows:

[0021] 1) Pretreatment of tussah silkworm chrysalis skin

[0022] For the separation of tussah silkworm chrysalis skin, wash the tussah silkworm chrysalis skin repeatedly with water until the pupa skin only contains white membranous matter, dry the water, and dry it in an oven at 60°C for 4 ...

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Abstract

The invention discloses a process utilizing enzymatic removing impurity to prepare tussah pupa skin chitosan, which is characterized by comprising steps: pretreatment of tussah pupa skins, removal of inorganic salts, protein hydrolysate, decoloration, preparation of chitosan through Chitin acetyl groups and purification of coarse chitosan and the like, and has the advantages that the process utilizing enzymatic removing impurity to prepare the tussah pupa skin chitosan is scientific and reasonable, short in production cycle, simple in process and less in waste water and waste liquid, does not damage environment and pollution, is easy to collect and treat products, uniform in product structure and high in molecular mass and wide in applied range.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a process for preparing chitosan from tussah silkworm chrysalis skin by using an enzymatic method to remove impurities. Background technique [0002] Tussah silkworm chrysalis is waste from the silk reeling and food processing industries. Its main components include inorganic salts, protein, and chitin. Chitin accounts for about 30% of the tussah chrysalis, and its content is considerable. It is a pity to discard it as waste. . After deacetylation of chitin, the resulting product is chitosan. Chitosan has a good effect on controlling bacteria, mold and other microorganisms, can reduce inflammation and stop acid; at the same time, it also has good biological functionality, blood compatibility, safety, microbial degradation and other excellent properties, which can effectively reduce Cholesterol and blood lipids. It is widely used in medicine, chemical industry, food, water treatment,...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCY02P20/582
Inventor 薛强刘隽彦
Owner JILIN SERICULTURE SCI RES INST
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