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Application of ift52 gene in diagnosis of osteoporosis

An osteoporosis and genetic technology, applied in disease diagnosis, microbial determination/examination, biochemical equipment and methods, etc. Mortality, genetic diagnosis and more

Active Publication Date: 2018-02-27
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The objects of auxiliary examination are generally patients with advanced osteoporosis, and cannot be used for the screening of patients with early osteoporosis

Method used

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  • Application of ift52 gene in diagnosis of osteoporosis
  • Application of ift52 gene in diagnosis of osteoporosis
  • Application of ift52 gene in diagnosis of osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Screening of genes differentially expressed in patients with osteoporosis and normal people

[0050] 1. Research object:

[0051] Osteoporosis group: randomly select 10 patients with primary osteoporosis admitted to the orthopedics department of the hospital, 5 males and 5 females, the youngest is 50 years old and the oldest is 75 years old. Inclusion criteria: in line with the "Recommended Diagnostic Criteria for Osteoporosis in China" (Second Draft). No obvious heart, liver, kidney, pulmonary insufficiency, no various endocrine diseases causing secondary osteoporosis, no tumor, diabetes and other serious diseases that interfere with bone metabolism.

[0052] Normal group: select 10 healthy volunteers aged 50-75, 5 males and 5 males.

[0053] There was no significant difference in age and gender between the two groups (P>0.10), which were comparable.

[0054] All research subjects were informed about this study and signed the informed consent.

[0055] 2....

Embodiment 2

[0073] Example 2 QPCR experiment verification of genes differentially expressed in patients with osteoporosis and normal people

[0074] 1. Research object:

[0075] Osteoporosis group: Randomly select 50 patients with primary osteoporosis admitted to the orthopedics department of the hospital, 25 males and 25 females, the youngest is 50 years old and the oldest is 75 years old. Inclusion criteria: in line with the "Recommended Diagnostic Criteria for Osteoporosis in China" (second draft). No obvious heart, liver, kidney, pulmonary insufficiency, no various endocrine diseases causing secondary osteoporosis, no tumor, diabetes and other serious diseases that interfere with bone metabolism.

[0076] Normal group: 46 healthy volunteers aged 50-75 were selected, 23 males and 23 males.

[0077] There was no significant difference in age and sex between the two groups (P>0.10), which were comparable.

[0078] All research subjects were informed about this study and signed the inf...

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PUM

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Abstract

The invention discloses a molecular marker for IFT52 genes used for early diagnosis of osteoporosis. By the molecular marker, expression conditions of differential genes of small sample research objects by high-throughput sequencing, and differential expression genes in large samples are verified by QPCR (quantitative polymerase chain reaction). A research result shows that whether a testee suffers from the osteoporosis or not is judged by detecting IFT52 gene expression conditions in blood. The detecting method can be used for early diagnosis of the osteoporosis, and has characteristics of timeliness and accuracy.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, and relates to a molecular marker for diagnosing osteoporosis, in particular to the application of the molecular marker-IFT52 gene in blood in the preparation of products for diagnosing osteoporosis. Background technique [0002] Osteoporosis (osteoporosis, OP) is a group of bone diseases caused by a variety of reasons. There is normal calcification in bone tissue, and the ratio of calcium salt to matrix is ​​normal. It is a metabolic bone disease characterized by a decrease in the amount of bone tissue per unit volume. . In most cases of osteoporosis, the loss of bone tissue is mainly due to increased bone resorption. Characterized by bone pain and susceptibility to fractures. [0003] The World Health Organization has listed osteoporosis as the second largest disease that endangers human health after cardiovascular disease. According to the latest "2013 China Osteoporotic Fracture Preventi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2800/108
Inventor 肖枫杨承刚
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD