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Glucagon detection kit and detection method thereof

A technology of glucagon and detection kit, which is applied in the field of glucagon detection kit and its detection, can solve the problem that the content of glucagon has not been reported, and achieve saving incubation time, high accuracy and temperature less demanding effect

Inactive Publication Date: 2015-12-02
GUANGZHOU JINDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, the ELISA kit for quantitatively detecting the glucagon content in the patient's blood has not been reported in China.

Method used

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  • Glucagon detection kit and detection method thereof
  • Glucagon detection kit and detection method thereof
  • Glucagon detection kit and detection method thereof

Examples

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Embodiment Construction

[0061] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The specific experimental methods not mentioned in the following examples are generally carried out according to conventional experimental methods.

[0062] A glucagon detection kit, comprising: 1) an enzyme-labeled plate coated with an antibody to glucagon; 2) a horseradish peroxidase-labeled glucagon detection antibody; 3) an enzyme-labeled antibody diluted 4) glucagon calibrator; 5) washing solution; 6) enzyme substrate solution;

[0063] The glucagon antibody is a monoclonal antibody that can specifically recognize the N-terminal of human glucagon. The antibody coating concentration is 2 μg / mL, and the coating volum...

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Abstract

The invention discloses a glucagon detection kit. The glucagon detection kit comprises a glucagon-antibody-coated solid-phase carrier (1), a horse-radish-peroxidase-labeled glucagon detection antibody (2), an enzyme-labeled antibody diluent (3), a glucagon calibrator (4), a washing liquid (5) and an enzyme substrate solution (6), wherein the glucagon antibody can specifically identify an N-terminal monoclonal antibody of human glucagon; and the glucagon detection antibody can specifically identify a C-terminal monoclonal antibody of human glucagon. The invention also discloses a detection method of the glucagon detection kit. The kit is simple in formula and convenient to use, and can sensitively, quickly and accurately measure glucagons in both blood and plasma at the room temperature in clinical test.

Description

technical field [0001] The invention belongs to the field of glucagon detection, in particular to a glucagon detection kit and a detection method thereof. Background technique [0002] Diabetes mellitus is a syndrome of metabolic disorders characterized by chronically elevated blood sugar levels. Insulin and glucagon are important for maintaining proper blood sugar levels. Insulin acts on the liver and peripheral tissues by increasing peripheral uptake of glucose and reducing glucose output from the liver to lower blood glucose levels, while glucagon binds to glucagon receptors on liver cells, causing hepatic glycogen Breakdown releases glucose stored in the form of glycogen, which helps maintain glucose levels in the blood. As these stores are depleted, glucagon stimulates the liver to synthesize additional glucose via gluconeogenesis. This glucose is released into the bloodstream, preventing hypoglycemia from occurring. [0003] Glucagon plays an important role in clini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535G01N33/577
CPCG01N33/535G01N33/577
Inventor 李民友陈亮李涛张玲姚汝荣蔺涛李妙红
Owner GUANGZHOU JINDE BIOTECH
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