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Microorganism restoration method for soil polluted by heavy metal

A technology for microbial remediation and contaminated soil, which is applied in the field of microbial remediation of heavy metal-contaminated soil, and can solve problems that have not been reported

Active Publication Date: 2015-12-09
山东省神农生态科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been studies on the adsorption of heavy metals in water by magnetotactic bacteria at home and abroad, but there is no report on the remediation of heavy metal-contaminated soil by magnetotactic bacteria.

Method used

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  • Microorganism restoration method for soil polluted by heavy metal
  • Microorganism restoration method for soil polluted by heavy metal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A microbial remediation method for heavy metal contaminated soil, comprising the following steps:

[0060] Step 1, isolating magnetotactic bacteria:

[0061] 1) Take a sterilized and dried petri dish, and set a barbed wire in the petri dish, take part of the sludge at 0.5cm below the water of Jizhuangzi Sewage Treatment Plant in Tianjin, put it into the petri dish, and cover the barbed wire;

[0062] 2) Insert a rubber plug into the sampling hole 2 of the magnetotactic bacteria collector, sterilize, fill the sterilized magnetotactic bacteria collector with sterile water, and cover the opening end of the magnetotactic bacteria collector Layer filter paper, and quickly buckled on the wire mesh;

[0063] 3) Put a magnet at the bottom of the magnetotactic bacteria collector with the N pole facing up, and after standing for 4 hours, collect magnetotactic bacteria from sampling hole 2 with a sterile syringe.

[0064] Step 2, cultivation of magnetotactic bacteria:

[0065] ...

Embodiment 2

[0074] A microbial remediation method for heavy metal-contaminated soil is provided in this embodiment, which specifically includes the following steps:

[0075] Step 1, isolating magnetotactic bacteria:

[0076] 1) Take a sterilized and dried petri dish, and set a barbed wire in the petri dish, take a part of the sludge at 1.0 cm from the activated sludge of Nanjing Suojin Village Sewage Treatment Plant, put it into the petri dish, and cover the barbed wire;

[0077] 2) Insert a rubber plug into the sampling hole 2 of the magnetotactic bacteria collector, sterilize, fill the sterilized magnetotactic bacteria collector with sterile water, and cover the opening end of the magnetotactic bacteria collector Layer filter paper, and quickly buckled on the wire mesh;

[0078] 3) Put a magnet at the bottom of the magnetotactic bacteria collector with the N pole facing up, and after standing for 5 hours, collect magnetotactic bacteria from sampling hole 2 with a sterile syringe.

[0...

Embodiment 3

[0089] A microbial remediation method for heavy metal-contaminated soil is provided in this embodiment, which specifically includes the following steps:

[0090] Step 1, isolating magnetotactic bacteria:

[0091] 1) Take a sterilized and dried petri dish, and set a barbed wire in the petri dish, take part of the sludge at 0.8cm below the water of Tianjin Jizhuangzi Sewage Treatment Plant, put it into the petri dish, and cover the barbed wire;

[0092] 2) Insert a rubber plug into the sampling hole 2 of the magnetotactic bacteria collector, sterilize, fill the sterilized magnetotactic bacteria collector with sterile water, and cover the opening end of the magnetotactic bacteria collector Layer filter paper, and quickly buckled on the wire mesh;

[0093] 3) Put a magnet at the bottom of the magnetotactic bacteria collector, with the N pole facing up, and let it stand for 4.5

[0094] Finally, the magnetotactic bacteria were collected from sampling hole 2 with a sterile syringe...

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Abstract

The invention provides a microorganism restoration method for soil polluted by heavy metal. The microorganism restoration method comprises the following steps of 1, separation of magnetotactic bacteria; 2, cultivation of the magnetotactic bacteria; 3, restoration of the soil polluted by the heavy metal through the magnetotactic bacteria; 4, recovery of the magnetotactic bacteria; and 5, separation of the magnetotactic bacteria and the heavy metal. The microorganism restoration method for the soil polluted by the heavy metal can effectively reduce the content of the heavy metal in the soil, thereby reducing the accumulation amount of the heavy metal in plants. The microorganism restoration method for the soil polluted by the heavy metal belongs to in-situ restoration, and is easy to operate, low in cost and good in restoration effect; in addition, the microorganism restoration method does not damage soil fertility and secondary pollution to the soil is avoided.

Description

technical field [0001] The invention belongs to the field of soil microbial remediation, and relates to microbial in-situ remediation, in particular to a microbial remediation method for heavy metal polluted soil. Background technique [0002] Soil pollution is caused by excessive harmful substances contained in the soil, exceeding the self-purification capacity of the soil, which causes changes in the composition, structure and function of the soil. Harmful substances gradually accumulate in the soil, through "soil→plant→human body", Or it can be indirectly absorbed by the human body through "soil → water → human body", reaching the level of endangering human health. Soil heavy metal pollution mainly includes mercury (Hg), cadmium (Cd), lead (Pb), chromium (Cr) and metalloid arsenic (As) with significant biological toxicity, as well as zinc (Zn), copper ( Cu), nickel (Ni) and other elements, heavy metal pollution elements have poor mobility in the soil, long residence time...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 马新攀
Owner 山东省神农生态科技股份有限公司
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