Plant disease resistance evaluation method, root injuring method and inoculation method

Abstract

The invention discloses a plant disease resistance evaluation method, a root injuring method and an inoculation method and belongs to the technical field of plant disease resistance evaluation. The root injuring method comprising the following steps of planting seedlings processed via disinfection and immersion into culturing substrate in a seedling nursing bag, making the seedlings grow via fertilizing application and daily care till the required size, taking the seedling nursing bag out of the culturing substrate arranged outside the bag, wherein the culturing substrate is cultured outside the seedling nursing bag; the seedling nursing bag is weaved by non-woven fabric; the root parts of the plants are exposed out of the seedling nursing bag and grow in the culturing substrate outside the seedling nursing bag; water is watered outside the seedling nursing bag; and the roots exposed out of the seedling nursing bag are pulled apart and root injury is finished. Root injury steps are simplified by the use of the method, effects on plant growth by root injury are reduced, and evaluation result for disease resistance cannot be affected.

Description

technical field [0001] The invention relates to the technical field of plant disease resistance identification, in particular to a plant disease resistance identification method, root injury method and inoculation method. Background technique [0002] Plant diseases have seriously affected the development of related planting industries. In particular, crop diseases are seriously harmful to my country's agricultural development. Taking cotton as an example, cotton verticillium wilt is one of the most important diseases in cotton production. Known as the "cancer" of cotton, it has seriously hindered the healthy and sustainable development of my country's cotton industry. Verticillium dahliae Kleb. is the causative bacterium of Verticillium dahliae, which has a wide range of hosts and strong variability, which brings great difficulties to the prevention and control of the disease. At present, planting disease-resistant varieties is still the most cost-effective control method...

AI Technical Summary

Problems solved by technology

The method of transplanting seedlings in seedling trays and dipping roots requires a transplanting process. After inoculation, it needs to go through a slow seedling stage. During the slow seedling stage, the disease resistance of cotton seedlings decreases, which may aggravate the disease, and the leaf loss caused by transplanting has brought a lot to the disease investigation. interference

Bottomless paper bowls or plastic bowls need to be made, and a disease resistance identification cycle is generally 40-50 days. Paper bowls are easy to rot and are not suitable for operation