Aptamer-modified magnetic nano material and application thereof in separating ochratoxin A

A technology of ochratoxin and aptamer, which is applied in the field of separation of ochratoxin A, can solve the problems of large OTA error and poor accuracy, and achieve the effects of improved accuracy, good selectivity, and high immobilization capacity

Inactive Publication Date: 2015-12-16
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes an improved method called this new type of material (the compound) made up of magnetite particles attached onto a special substrate or coating inside another part). These materials help bind other molecules like proteins better than their own counterparts when they come into contact with certain chemicals found naturally within our body's bloodstream. They allow us to detect minute amounts of these harmful agents more efficiently and accurately compared against existing methods such as Western blotting analysis.

Problems solved by technology

Technologies exist today related to identifying chemicals called cholesterols found within cells through their ability to attach themselves to various proteins involved in cellular functions like calcium transport systems. These techniques involve analyzing these compounds using special agents made up mostly of small organisms containing nucleators. However, there currently exists no reliable way to accurately determine if some particular compound(s), known as oxysalenictaene A ("Oxytoxicoan"), occurs naturally occurring throughout humans' bodies due to its presence at very low levels in plants and animals.

Method used

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  • Aptamer-modified magnetic nano material and application thereof in separating ochratoxin A
  • Aptamer-modified magnetic nano material and application thereof in separating ochratoxin A
  • Aptamer-modified magnetic nano material and application thereof in separating ochratoxin A

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Embodiment 1

[0045] This embodiment provides an ochratoxin A aptamer-magnetic nanoparticle composite, which includes an inner core layer and an outer shell layer, wherein the inner core layer is made of paramagnetic Fe 3 O 4 It is composed of magnetic nanoparticles, and the outer shell layer includes an inner silanization layer and an aptamer modification layer on the outer side of the silanization layer.

[0046] The ochratoxin A aptamer-magnetic nanoparticle complex is prepared by the following method:

[0047] (1) Preparation of the inner core layer

[0048] 1.99gFeCl 2 ·4H 2 O and 5.41gFeCl 3 ·6H 2 O was dissolved in 160ml of 0.1mol / L hydrochloric acid solution to obtain a mixed solution. The FeCl 2 ·4H 2 O and FeCl 3 ·6H 2 The molar ratio of O is 1:2;

[0049] The mixed solution was transferred into a 250ml flask, and nitrogen was introduced. Under nitrogen protection, the mixture was stirred vigorously for 15 minutes. At 30°C, 3mol / L NaOH solution was slowly added dropwise to the mixed soluti...

Embodiment 2

[0079] This embodiment provides an ochratoxin A aptamer-magnetic nanoparticle composite, which includes an inner core layer and an outer shell layer, wherein the inner core layer is made of paramagnetic Fe 3 O 4 It is composed of magnetic nanoparticles, and the outer shell layer includes an inner silanization layer and an aptamer modification layer on the outer side of the silanization layer.

[0080] The ochratoxin A aptamer-magnetic nanoparticle complex is prepared by the following method:

[0081] (1) Preparation of the inner core layer

[0082] 1.99gFeCl 2 ·4H 2 O and 5.41gFeCl 3 ·6H 2 O was dissolved in 160ml of 0.1mol / L hydrochloric acid solution to obtain a mixed solution. The FeCl 2 ·4H 2 O and FeCl 3 ·6H 2 The molar ratio of O is 1:2;

[0083] The mixed solution was transferred into a 250ml flask, and nitrogen was introduced. Under the protection of nitrogen, the mixture was stirred vigorously for 30 minutes. At 50°C, 5mol / L NaOH solution was slowly added dropwise to the mixed...

Embodiment 3

[0098] This embodiment provides an ochratoxin A aptamer-magnetic nanoparticle composite, which includes an inner core layer and an outer shell layer, wherein the inner core layer is made of paramagnetic Fe 3 O 4 It is composed of magnetic nanoparticles, and the outer shell layer includes an inner silanization layer and an aptamer modification layer on the outer side of the silanization layer.

[0099] The ochratoxin A aptamer-magnetic nanoparticle complex is prepared by the following method:

[0100] (1) Preparation of the inner core layer

[0101] 1.99gFeCl 2 ·4H 2 O and 5.41gFeCl 3 ·6H 2 O was dissolved in 160ml of 0.1mol / L hydrochloric acid solution to obtain a mixed solution. The FeCl 2 ·4H 2 O and FeCl 3 ·6H 2 The molar ratio of O is 1:2;

[0102] The mixed solution was transferred into a 250ml flask, and nitrogen was introduced. Under nitrogen protection, the mixture was stirred vigorously for 25 min. At 40°C, 4mol / L NaOH solution was slowly added dropwise to the mixed solution a...

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Abstract

The invention provides an aptamer-magnetic nanoparticle compound, which comprises an inner nuclear layer formed by paramagnetic Fe3O4 magnetic nanoparticles, a silanization layer and an aptamer modification layer located at the outer side of the silanization layer, so that the aptamer-magnetic nanoparticle compound not only has a characteristic of being quickly separated from a complex sample solution under an externally applied magnetic field, but also has a characteristic of carrying out specificity recognition capture on a detected substance by using an aptamer. Therefore, the detected substance can be separated from the complex sample solution quickly and effectively by using the aptamer-magnetic nanoparticle compound provided by the invention, the selectivity is good, and the separation efficiency is high. Data shows that the separation efficiency and the recovery rate of the aptamer-magnetic nanoparticle compound reach up to 80-92%. Furthermore, the separated detected substance is gathered and then applied to detection again, thereby being capable of effectively improving the accuracy.

Description

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Claims

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Application Information

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Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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