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Culturing method for strawberry fruit calluses

A callus, culture method technology, applied in the field of plant biology, can solve problems such as not meeting research needs

Active Publication Date: 2015-12-23
JIANGSU ZHONGJIANG SEED CO LTD
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] At present, the research on callus focuses on the callus of leaves. However, with the deepening of strawberry molecular biology research, it is obviously not enough to study the callus culture of a single organ or tissue.

Method used

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  • Culturing method for strawberry fruit calluses
  • Culturing method for strawberry fruit calluses
  • Culturing method for strawberry fruit calluses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] The plant material is respectively selected as the fruit of 'Hongyan' strawberry 5-8 days, 15-18 days, 23-25, 28-30 days after flowering, and the cultivation method of the fruit callus is operated according to the following steps.

[0019] 1. Explant treatment

[0020] First, the surface of the fruit is rinsed with running water. Under aseptic conditions, it is sterilized with 75% ethanol for 2 minutes, then rinsed with sterile deionized water for 3 times, then sterilized with 10% sodium hypochlorite for 8 minutes, and then disinfected with sterile deionized water Rinse 4 to 5 times, and then use sterile filter paper to dry the surface moisture of the peel. After the disinfection is completed, carefully peel off the peel with a sterile scalpel blade, cut the pulp of the core into 0.5 mm thin slices, punch the pulp into discs with a 0.5 cm diameter puncher, and inoculate the prepared callus in the induction medium.

[0021] 2. Explant culture

[0022] After culturing ...

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Abstract

The invention discloses a culturing method for strawberry fruit calluses. First, strawberry fruits are sterilized and cultured for 5 days at 25 DEG C inside a dark culture box without illumination, then irregular-shape calluses will grow around the fruit original pieces, the calluses on the edges of fruit pulp are cut off under sterile conditions to be inoculated into a callus proliferation culture medium and then cultured for 10-15 days, and then the size of the calluses is 3-4 times the original size. The induction rate of the fruit calluses can reach 100%, a new path is provided for strawberry molecular biology and gene engineering research, a new test material is provided for strawberry gene engineering research and gene transformation, and reference is provided for culturing of the strawberry fruit calluses.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a callus culture method. Background technique [0002] At present, many countries have carried out research on strawberry genome. The direction of strawberry genome research mainly involves several major aspects such as plant resistance, fruit quality and fruit production characteristics. Among them, the important economic traits of strawberry, especially the molecular mechanism related to the formation of fruit quality Research has also become a hot spot in strawberry genome research. In the study of the molecular mechanism of strawberry, callus has become a commonly used test material, and it is also a necessary test material for gene transformation in genetic engineering research. Callus refers to a group of apolar, vigorously dividing parenchyma cell clusters formed by explants under in vitro culture conditions. During the culture process, they can develop into complete re...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王媛花张立伟颜志明王全智
Owner JIANGSU ZHONGJIANG SEED CO LTD
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