Fresh semen artificial insemination method for improving donkey conception rate

Abstract

The invention discloses a fresh semen artificial insemination method for improving the donkey conception rate. The method comprises a fresh semen collecting, disinfecting and diluting process and an induced ovulation and timed semen deposition process; firstly, collected seminal fluid is disinfected and diluted; then, a donkey can ovulate in the concentrated time by using a hormone injection method; the atresia probability in the follicular development process is reduced; disinfected and diluted fresh semens are subjected to artificial semen deposition within 10 hours before the ovulation and within 6 hours after the ovulation, and the semen deposition dose is 15mL; donkey semens and donkey ova are enabled to meet to complete the insemination process in the time period with insemination capability; and the donkey conception is realized. An induced ovulation and timed semen deposition technical measure is adopted, so that the conception rate in the estrus can reach 73.3 percent.

Description

technical field [0001] The invention relates to a method for artificial insemination of livestock. technical background [0002] Donkeys belong to the genus Equus. In order to increase the breeding capacity, artificial insemination is generally adopted. Due to the particularity of the ovarian structure of equine animals, the duration of estrus changes greatly after the appearance of estrus, which generally lasts for 3 to 14 days. There is a big difference, the ovulation time is mostly on the 7th to 10th day of estrus, and the ovulation varies from 12 to 24 hours before the cessation of estrus, which makes it difficult to determine the time of donkey insemination. The traditional technique is through rectal palpation of the ovaries, and insemination is performed when the follicles are developed until the follicles are soft, elastic and fluctuating, that is, the third and fourth stages of rectal examination, but the rectal palpation technique requires technicians A long time...

AI Technical Summary

Problems solved by technology

Due to the particularity of the ovarian structure of equine animals, the duration of estrus changes greatly after the appearance of estrus, which generally lasts for 3 to 14 days. There is a large difference, the ovulation time is mostly on the 7th to 10th day of estrus, and the ovulation varies from 12 to 24 hours before the cessation of estrus, which makes it difficult to determine the time of donkey insemination

The traditional technique is through rectal palpation of the ovary, and insemination is performed when the follicles are developed until the follicles are soft, elastic, and fluctuating, that is, the third and fourth stages of rectal examination, but the rectal palpation technique requires technicians A long time of experience has accumulated, and different people have different judgments on the stage of follicle development. Even if the stage of follicle development is accurately determined, it is difficult to accurately determine the time of ovulation. Follicles at stage 3 will last for 1 to 3 days, and follicles at stage 4 will last for 1 to 3 days. It will also last for 1-3 days, and it is difficult to determine the ovulation time, which makes it difficult to control estrus ovulation. The conception rate after one artificial insemination is less than 30%, and it is necessary to perform multiple artificial inseminations on donkeys to increase the conception rate.

According to the test, artificial insemination is performed every two hours, and the conception rate can reach more than 70% through continuous operation for 1-3 days; such continuous operation is time-consuming and laborious, which is difficult to achieve under normal circumstances

Images