A kind of kombucha protein beverage and preparation method thereof
A protein beverage and kombucha technology, which is applied in the field of microbial fermentation to achieve the effects of ensuring stability, shortening fermentation time, and being beneficial to industrialized production
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Embodiment 1
[0024] 1. Production of bacteria powder
[0025] Pick the 2-3 rings of Kluyveromyces marxii strain BD1002 cultured on the PDA solid medium for 24-48h and insert it into the yeast medium, place it at 30°C, shake it at 180r / min for 18h, and press 1% (v The inoculation amount of / v) was transferred into 400ml yeast culture medium, and cultured with shaking at 180r / min for 48h. Pick the activated Lactobacillus acidophilus NCFM and Lactobacillus casei LC2W2-3 rings respectively into the MRS medium, anaerobic static culture at 37°C for 18 hours, then transfer to 400ml MRS at an inoculum size of 1% (v / v) In the culture medium, at 37°C, anaerobic static culture was carried out for 48 hours.
[0026] The fermentation broth obtained from the above cultivation was centrifuged at 12000 rpm for 10 min under aseptic conditions to collect the bacteria. Wash twice with physiological saline 0.9% (w / v) NaCl, resuspend in 20ml skim milk (10% w / v, 115°C, 15min sterilization), pre-freeze in -80°...
Embodiment 2
[0033] 1. Production of bacteria powder
[0034] Pick the 2-3 rings of Kluyveromyces marxii strain BD1002 cultured on the PDA solid medium for 24-48h and insert it into the yeast medium, place it at 30°C, shake it at 180r / min for 18h, and press 1% (v The inoculation amount of / v) was transferred into 400ml yeast culture medium, and cultured with shaking at 180r / min for 48h. Pick the activated Lactobacillus acidophilus NCFM and Lactobacillus casei LC2W2-3 rings respectively into the MRS medium, anaerobic static culture at 37°C for 18 hours, then transfer to 400ml MRS at an inoculum size of 1% (v / v) In the culture medium, at 37°C, anaerobic static culture was carried out for 48 hours.
[0035] The fermentation broth obtained from the above cultivation was centrifuged at 12000 rpm for 10 min under aseptic conditions to collect the bacteria. Wash twice with physiological saline 0.9% (w / v) NaCl, resuspend in 20ml skim milk (10% w / v, 115°C, 15min sterilization), pre-freeze in -80°...
Embodiment 3
[0042] 1. Production of bacteria powder
[0043] Pick the 2-3 rings of Kluyveromyces marxii strain BD1002 cultured on the PDA solid medium for 24-48h and insert it into the yeast medium, place it at 30°C, shake it at 180r / min for 18h, and press 1% (v The inoculation amount of / v) was transferred into 400ml yeast culture medium, and cultured with shaking at 180r / min for 48h. Pick the activated Lactobacillus acidophilus NCFM and Lactobacillus casei LC2W2-3 rings respectively into the MRS medium, anaerobic static culture at 37°C for 18 hours, then transfer to 400ml MRS at an inoculum size of 1% (v / v) In the culture medium, at 37°C, anaerobic static culture was carried out for 48 hours.
[0044] The fermentation broth obtained from the above cultivation was centrifuged at 12000 rpm for 10 min under aseptic conditions to collect the bacteria. Wash twice with physiological saline 0.9% (w / v) NaCl, resuspend in 20ml skim milk (10% w / v, 115°C, 15min sterilization), pre-freeze in -80°...
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