Separation method of soybean leaf protoplast for subcellular localization
A technology for protoplast and plasmolysis separation, applied to plant cells, etc., can solve the problem that plant genotype has a great influence on protoplast separation, and achieve the effect of easy acquisition and simple enzymatic hydrolysis method
Active Publication Date: 2016-01-06
ANHUI SCI & TECH UNIV
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[0018] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with examples. These examples are only used to explain the present invention, and do not constitute a limitation to the protection scope of the present invention.
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The invention relates to a separation method of soybean leaf protoplast for subcellular localization. The method includes: using tender leaves of soybean Xiangdou No.3 as a material, and by controlling factors such as plasmolysis separation time, ratio and concentrations of cellulase Onozuka R-10, hemicellulase, macerozyme R-10 and pectolyase Y-23, concentration of mannitol in enzymatic hydrolysate, enzymatic hydrolysate pH and enzymolysis time, screening out most suitable conditions for the separation of soybean leaf protoplast of Xiangdou No.3. The soybean leaf protoplast obtained by using the method is abundant and higher in activity and is effective in subcellular localization, and the method allows simple and quick separation of the soybean leaf protoplast of Xiangdou No.3.
Description
technical field [0001] The invention relates to a soybean leaf protoplast separation method for subcellular localization, which belongs to the field of biotechnology. Background technique [0002] The plant genotype has a great influence on the isolation of protoplasts. Xiangdou No. 3 soybean is the main soybean material in our research, but the method for the isolation of protoplasts from Xiangdou No. 3 soybean has not been reported yet. Using the young leaves of soybean variety Xiangdou No. 3 as materials, we established a leaf protoplast isolation system of Xiangdou No. 3. This invention will provide important technical support for our research on transient gene expression and subcellular localization of proteins. Contents of the invention [0003] Purpose of the invention: the purpose of the invention is to provide a method for isolating soybean leaf protoplasts for subcellular localization. [0004] Technical solution: The present invention screens out the most suita...
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Inventor 舒英杰周玉丽陶源胡能兵何庆元黄守程
Owner ANHUI SCI & TECH UNIV