Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Assay method of morphologic particle size of drug carrying micelle

A technology of drug-loaded micelles and determination methods, which can be used in measuring devices, particle size analysis, particle and sedimentation analysis, etc., and can solve problems such as low density

Active Publication Date: 2016-01-13
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the shape of drug-loaded micelles, the density is generally small, and the particle size cannot be obtained by using a conventional CPS disc centrifugal nanoparticle size analyzer.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Assay method of morphologic particle size of drug carrying micelle
  • Assay method of morphologic particle size of drug carrying micelle
  • Assay method of morphologic particle size of drug carrying micelle

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0031] The preparation of DSPE-PEG drug-loaded micelles comprises the following steps:

[0032] First take a certain amount of DSPE-PEG (phosphatidylethanolamine-polyethylene glycol) reagent in the water phase and add a small amount of glucose as a stabilizer to make DSPE-PEG form micelles, then add the hydrophobic drug doxorubicin, and keep stirring until the solution Clarification, that is, the formation of drug-loaded micelles coated with doxorubicin drug.

preparation example 2

[0034] The preparation of DSPE-PEG drug-loaded micelles comprises the following steps:

[0035] First take a certain amount of DSPE-PEG (phosphatidylethanolamine-polyethylene glycol) reagent in the water phase and add a small amount of glucose as a stabilizer to make DSPE-PEG form micelles, then add the drug irinotecan hydrochloride, and keep stirring until the solution is clear , that is, to form drug-loaded micelles coated with irinotecan hydrochloride.

preparation example 3

[0037] The preparation of DSPE-PEG drug-loaded micelles comprises the following steps:

[0038] First take a certain amount of DSPE-PEG (phosphatidylethanolamine-polyethylene glycol) reagent in the water phase and add a small amount of glucose as a stabilizer to make DSPE-PEG form micelles, then add the drug flurbiprofen, and keep stirring until the solution is clear , that is, to form drug-loaded micelles coated with flurbiprofen drug.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
particle sizeaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to an assay method of the morphologic particle size of a drug carrying micelle, which comprises the following steps: (1) prepare a cesium chloride gradient solution with average density being [rou]p; 2) successively injecting the cesium chloride gradient solution into a low-density extension disk of a CPS disk centrifugal-type nano granularity analyzer from high concentration to low concentration to establish gradient; (2) when the gradient of the cesium chloride gradient solution is stable, loading a sample of a to-be-test drug carrying micelle solution from edge of the low-density extension disk to the CPS centrifugal granularity analyzer to determine the sedimentation rate of the to-be-test drug carrying micelle, thereby obtaining the morphologic particle size of the drug carrying micelle. In the method, the density gradient centrifugal method is employed to test the morphologic particle size of the drug carrying micelle, wherein a defect of low density of the drug carrying micelle is overcome through change of rotation speed and gradient solution concentration, thereby accurately assaying the morphologic particle size of the drug carrying micelle.

Description

technical field [0001] The invention belongs to the field of measuring the shape of drug-loaded micelles, and relates to a method for measuring the shape and particle size of drug-loaded micelles, in particular to a method for measuring the shape and particle size of drug-loaded micelles using a CPS disc centrifugal nanoparticle size analyzer. method. Background technique [0002] Polymeric micelles are drug carriers in the nanometer range. Compared with liposomes, nanospheres and other drug carriers, they have the following advantages: ①Macromolecular micelles have good biocompatibility, and micelles can effectively isolate drugs and The external medium weakens the impact of drugs with large side effects on the human body; ②Because the macromolecular micelles are protected by the hydrophilic part outside, it is not easy to adsorb and recognize proteins and phagocytes, ensuring its residence time in plasma and tissues , long circulation time in vivo, good stability; ③Macrom...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/02
Inventor 葛广路王瑞敏梁兴杰车晶
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com