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A kind of assay method of drug-loaded micelle morphology particle size

A technology of drug-loaded micelles and determination methods, which can be used in measurement devices, particle size analysis, particle and sedimentation analysis, etc., and can solve problems such as low density

Active Publication Date: 2016-10-26
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the shape of drug-loaded micelles, the density is generally small, and the particle size cannot be obtained by using a conventional CPS disc centrifugal nanoparticle size analyzer.

Method used

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  • A kind of assay method of drug-loaded micelle morphology particle size
  • A kind of assay method of drug-loaded micelle morphology particle size
  • A kind of assay method of drug-loaded micelle morphology particle size

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0031] The preparation of DSPE-PEG drug-loaded micelles comprises the following steps:

[0032] First take a certain amount of DSPE-PEG (phosphatidylethanolamine-polyethylene glycol) reagent in the water phase and add a small amount of glucose as a stabilizer to make DSPE-PEG form micelles, then add the hydrophobic drug doxorubicin, and keep stirring until the solution Clarification, that is, the formation of drug-loaded micelles coated with doxorubicin drug.

preparation example 2

[0034] The preparation of DSPE-PEG drug-loaded micelles comprises the following steps:

[0035] First take a certain amount of DSPE-PEG (phosphatidylethanolamine-polyethylene glycol) reagent in the water phase and add a small amount of glucose as a stabilizer to make DSPE-PEG form micelles, then add the drug irinotecan hydrochloride, and keep stirring until the solution is clear , that is, to form drug-loaded micelles coated with irinotecan hydrochloride.

preparation example 3

[0037] The preparation of DSPE-PEG drug-loaded micelles comprises the following steps:

[0038] First take a certain amount of DSPE-PEG (phosphatidylethanolamine-polyethylene glycol) reagent in the water phase and add a small amount of glucose as a stabilizer to make DSPE-PEG form micelles, then add the drug flurbiprofen, and keep stirring until the solution is clear , that is, to form drug-loaded micelles coated with flurbiprofen drug.

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Abstract

The present invention relates to a kind of assay method of drug-loaded micelle form particle size, described method comprises the following steps: (1) preparation cesium chloride gradient solution, the average density of described cesium chloride gradient solution is ρ p ; Inject the cesium chloride gradient solution into the low density expansion disc of the CPS disc centrifugal nanoparticle size analyzer successively from high concentration to low concentration to establish a gradient; (2) after the gradient solution gradient is stable, the drug-loaded micelles to be tested are The solution was loaded from the edge of the low-density expansion plate to the CPS centrifugal particle size analyzer, and the sedimentation rate of the drug-loaded micelles to be tested was measured to obtain the morphological particle size of the drug-loaded micelles. The present invention adopts the density gradient centrifugation method to test the morphological particle size of the drug-loaded micelles, and overcomes the shortcoming of the low density of the drug-loaded micelles by changing the rotational speed and the concentration of the gradient liquid, and accurately tests the morphological particle diameters of the drug-loaded micelles.

Description

technical field [0001] The invention belongs to the field of measuring the shape of drug-loaded micelles, and relates to a method for measuring the shape and particle size of drug-loaded micelles, in particular to a method for measuring the shape and particle size of drug-loaded micelles using a CPS disc centrifugal nanoparticle size analyzer. method. Background technique [0002] Polymeric micelles are drug carriers in the nanometer range. Compared with liposomes, nanospheres and other drug carriers, they have the following advantages: ①Macromolecular micelles have good biocompatibility, and micelles can effectively isolate drugs and The external medium weakens the impact of drugs with large side effects on the human body; ②Because the macromolecular micelles are protected by the hydrophilic part outside, it is not easy to adsorb and recognize proteins and phagocytes, ensuring its residence time in plasma and tissues , long circulation time in vivo, good stability; ③Macrom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/02
Inventor 葛广路王瑞敏梁兴杰车晶
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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