Biological antidiarrheal agent for pigs and preparation method and application thereof

A biological and antidiarrheal technology, which is applied in the preparation of biological antidiarrheal agents, animal health care products, and biological health care products for pigs, and can solve the problems that the therapeutic effect has not been studied in depth.

Active Publication Date: 2016-01-27
北京昕大洋科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of probiotics in this invention focuses on the use of probiotics to adjust the intestinal flora structure of poultry and livestoc

Method used

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  • Biological antidiarrheal agent for pigs and preparation method and application thereof
  • Biological antidiarrheal agent for pigs and preparation method and application thereof
  • Biological antidiarrheal agent for pigs and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of the original bacterial powder of Bacillus licheniformis DE, Bacillus subtilis K018 and Saccharomyces cerevisiae XDN-1188:

[0047] According to the microbial fermentation process, the DE strain of Bacillus licheniformis preserved on the slant was first activated and expanded with beef extract peptone medium, and then inoculated into the fermenter aseptically by the pressure difference method according to the inoculum amount of 15mL / T. The pH is 6.5, the temperature is controlled at 34°C, and fermented for 38 hours to obtain a fermented liquid; similarly, the Bacillus subtilis K018 strain is also activated and expanded with beef extract peptone medium, and the inoculation amount is 13mL / T, and the differential pressure method is used. Aseptically inoculate into a fermenter, the initial pH is 6.0, the temperature is controlled at 33° C., and fermented for 51 hours to obtain a fermented liquid. After culturing and activating Saccharomyces cerevisiae XDN-1188...

Embodiment 2

[0057] Preparation of the original bacterial powder of Bacillus licheniformis DE, Bacillus subtilis K018 and Saccharomyces cerevisiae XDN-1188:

[0058] According to the microbial fermentation process, the Bacillus licheniformis DE strain preserved on the slant was first activated and expanded with beef extract peptone medium, and then according to the inoculum amount of 13mL / T, it was aseptically inoculated into the fermenter by the differential pressure method. The pH is 6.5, the temperature is controlled at 33°C, and fermented for 40 hours to obtain a fermentation broth; similarly, the Bacillus subtilis K018 strain is also activated and expanded with beef extract peptone medium, and the inoculation amount is 10mL / T, and the differential pressure method is used to Aseptically inoculate into a fermenter, the initial pH is 6.0, the temperature is controlled at 34° C., and fermented for 50 hours to obtain a fermented liquid. After culturing and activating Saccharomyces cerevisi...

Embodiment 3

[0068] Preparation of the original bacterial powder of Bacillus licheniformis DE, Bacillus subtilis K018 and Saccharomyces cerevisiae XDN-1188:

[0069] According to the microbial fermentation process, the DE strain of Bacillus licheniformis preserved on the slant was first activated and expanded with beef extract peptone medium, and then inoculated into the fermenter aseptically by the differential pressure method according to the inoculum amount of 10mL / T. The pH is 6.5, the temperature is controlled at 35°C, and fermented for 36 hours to obtain the fermentation broth; similarly, the Bacillus subtilis K018 strain is also activated and expanded with beef extract peptone medium, and the inoculum amount is 15mL / T, and the differential pressure method is used. Aseptically inoculate into a fermenter, the initial pH is 6.0, the temperature is controlled at 32° C., and fermented for 53 hours to obtain a fermented liquid. After culturing and activating Saccharomyces cerevisiae XDN-1...

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Abstract

The invention relates to a biological antidiarrheal agent for pigs and a preparation method thereof and belongs to the technical field of animal healthcare products. The preparation method includes: selecting alkaline protease produced by fermenting Bacillus licheniformis CGMCC No.6102, brewer's yeast CGMCC No.11355, Bacillus subtilis CGMCC No.6101 and Bacillus licheniformis CGMCC No.11353, separated from a scavenger intestinal tract, which are collected strains, as main ingredients, wherein the Bacillus licheniformis DE is 15-30 billion cfu/g, the Bacillus subtilis K018 is 20-40 billion cfu/g, the brewer's yeast XDN-1188 is 5-15 billion cuf/g, nano montmorillonite is 200-300mg/g, the alkaline protease is 50-150U/g, and the biological antidiarrheal agent is prepared by taking glucose as a vector. The biological antidiarrheal agent can be used for daily breeding of pig industry, can be used as a raw material of feed and has quick treatment effect on piglet diarrhea.

Description

technical field [0001] The invention relates to animal health care products, in particular to a biological health care product for pigs that can quickly relieve diarrhea, and to a preparation method of the biological antidiarrheal agent, belonging to the technical field of animal health care products. Background technique [0002] Piglet diarrhea is a typical multifactorial disease in intensive pig farming. This disease is one of the most serious piglet diseases at present, and it is also an important cause of piglet death. According to the survey, the death of piglets due to diarrhea accounts for 39.8% of the total number of piglet deaths. In recent years, piglet diarrhea is very common in my country. According to reports, piglets below 30kg have an annual average incidence of 46.5% and a mortality rate of 10.3%. In countries where the pig industry is relatively developed, such as the United States, piglet diarrhea ranks first among the hazards of the pig industry, which s...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61P1/12A23K50/30A61K33/06A61K35/742A61K36/076
Inventor 郭宝林赵丽芳
Owner 北京昕大洋科技发展有限公司
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