Biological antidiarrheal agent for pigs and preparation method and application thereof
A biological and antidiarrheal technology, which is applied in the preparation of biological antidiarrheal agents, animal health care products, and biological health care products for pigs, and can solve the problems that the therapeutic effect has not been studied in depth.
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Embodiment 1
[0046] Preparation of the original bacterial powder of Bacillus licheniformis DE, Bacillus subtilis K018 and Saccharomyces cerevisiae XDN-1188:
[0047] According to the microbial fermentation process, the DE strain of Bacillus licheniformis preserved on the slant was first activated and expanded with beef extract peptone medium, and then inoculated into the fermenter aseptically by the pressure difference method according to the inoculum amount of 15mL / T. The pH is 6.5, the temperature is controlled at 34°C, and fermented for 38 hours to obtain a fermented liquid; similarly, the Bacillus subtilis K018 strain is also activated and expanded with beef extract peptone medium, and the inoculation amount is 13mL / T, and the differential pressure method is used. Aseptically inoculate into a fermenter, the initial pH is 6.0, the temperature is controlled at 33° C., and fermented for 51 hours to obtain a fermented liquid. After culturing and activating Saccharomyces cerevisiae XDN-1188...
Embodiment 2
[0057] Preparation of the original bacterial powder of Bacillus licheniformis DE, Bacillus subtilis K018 and Saccharomyces cerevisiae XDN-1188:
[0058] According to the microbial fermentation process, the Bacillus licheniformis DE strain preserved on the slant was first activated and expanded with beef extract peptone medium, and then according to the inoculum amount of 13mL / T, it was aseptically inoculated into the fermenter by the differential pressure method. The pH is 6.5, the temperature is controlled at 33°C, and fermented for 40 hours to obtain a fermentation broth; similarly, the Bacillus subtilis K018 strain is also activated and expanded with beef extract peptone medium, and the inoculation amount is 10mL / T, and the differential pressure method is used to Aseptically inoculate into a fermenter, the initial pH is 6.0, the temperature is controlled at 34° C., and fermented for 50 hours to obtain a fermented liquid. After culturing and activating Saccharomyces cerevisi...
Embodiment 3
[0068] Preparation of the original bacterial powder of Bacillus licheniformis DE, Bacillus subtilis K018 and Saccharomyces cerevisiae XDN-1188:
[0069] According to the microbial fermentation process, the DE strain of Bacillus licheniformis preserved on the slant was first activated and expanded with beef extract peptone medium, and then inoculated into the fermenter aseptically by the differential pressure method according to the inoculum amount of 10mL / T. The pH is 6.5, the temperature is controlled at 35°C, and fermented for 36 hours to obtain the fermentation broth; similarly, the Bacillus subtilis K018 strain is also activated and expanded with beef extract peptone medium, and the inoculum amount is 15mL / T, and the differential pressure method is used. Aseptically inoculate into a fermenter, the initial pH is 6.0, the temperature is controlled at 32° C., and fermented for 53 hours to obtain a fermented liquid. After culturing and activating Saccharomyces cerevisiae XDN-1...
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