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A technical method for producing Bacillus megaterium by using distiller's grains

A technology of Bacillus megaterium and distiller's grains is applied in the field of fermentation engineering to achieve the effects of low price, increased yield and high spore rate

Active Publication Date: 2022-03-11
四川润格生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, in the production of bio-organic fertilizers, Bacillus megaterium is only one of the mixed fermentation flora, and is often used together with Bacillus subtilis and other microorganisms. There is no report on the fermentation of Bacillus megaterium alone to prepare biological feed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A technical method for producing Bacillus megaterium by utilizing distiller's grains, comprising the following steps:

[0037] (1) Strain activation: Streak the Bacillus megaterium strain stored at -80°C on a solid seed medium plate, and culture it at a temperature of 38°C for 22 hours;

[0038](2) First-level seed cultivation: Pick three to five rings of activated Bacillus coagulans and inoculate them into the liquid seed medium, place them in a shaker, and culture them with shaking at a temperature of 380°C and 250rpm 23h, the first-grade seed fungus was obtained;

[0039] (3) Pretreatment of distiller's grains: mix distiller's grains and water evenly according to the mass ratio of 1:1.5, add sodium bicarbonate to adjust the pH value to 6.8, inoculate the first-grade seed bacteria, keep the ventilation rate at 23%, and the temperature at 33°C. Feed fermentation, cultured for 5 days, to obtain pretreated distiller's grains;

[0040] (4) preparation of fermentation me...

Embodiment 2

[0046] A technical method for producing Bacillus megaterium by utilizing distiller's grains, comprising the following steps:

[0047] (1) Strain activation: Streak the Bacillus megaterium strain preserved at -80°C on a solid seed medium plate, and cultivate it for 20 hours at a temperature of 40°C; the strain is Bacillus megaterium;

[0048] (2) First-level seed cultivation: pick three to five rings of activated Bacillus coagulans and inoculate them into the liquid seed medium, place them in a shaker, and culture them with shaking at a temperature of 40°C and 250rpm 20h, the first-grade seed fungus was obtained;

[0049] (3) Pretreatment of distiller's grains: mix distiller's grains and water evenly according to the mass ratio of 1:2, add sodium bicarbonate to adjust the pH value to 6.5, inoculate the first-grade seed fungus, keep the ventilation rate at 30%, and the temperature at 30°C. Fermentation and culture for 7 days to obtain pretreated distiller's grains;

[0050] (4...

Embodiment 3

[0056] A technical method for producing Bacillus megaterium by utilizing distiller's grains, comprising the following steps:

[0057] (1) Strain activation: Streak the Bacillus megaterium strain preserved at -80°C on a solid seed medium plate, and cultivate it at a temperature of 37°C for 24 hours; the strain is Bacillus megaterium;

[0058] (2) First-level seed cultivation: pick three to five rings of activated Bacillus coagulans and inoculate them into the liquid seed medium, place them in a shaker, and culture them with shaking at a temperature of 35°C and 250rpm 24h, the first-grade seed fungus was obtained;

[0059] (3) Pretreatment of distiller's grains: mix distiller's grains and water evenly according to the mass ratio of 1:1, add sodium bicarbonate to adjust the pH value to 7.0, inoculate the first-grade seed fungus, keep the ventilation rate at 15%, and the temperature at 35°C. Feed fermentation, cultured for 3 days, to obtain pretreated distiller's grains;

[0060...

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Abstract

The invention discloses a technical method for producing Bacillus megaterium by using distiller's grains, which belongs to the technical field of fermentation engineering and includes: activation of strains, primary seed cultivation, pretreatment of distiller's grains, preparation of fermentation medium, and solid fermentation to obtain megaspores Bacillus culture: the fermentation process of the present invention adopts the method of solid-state fermentation to produce Bacillus megaterium, and the whole fermentation process follows the aseptic operation procedure, so that the solid-state culture production of Bacillus megaterium realizes pure culture, and large-scale enlarged production can be realized. The fermentation medium involved in the present invention solves the problems of low number of viable bacteria in the fermentation of distiller's grains as the main raw material, and the problems of easy contamination of miscellaneous bacteria in liquid culture, low nutrient utilization rate, and difficult separation of bacteria and metabolites. Greatly increased the production of Bacillus megaterium.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a technical method for producing Bacillus megaterium by using distiller's grains. Background technique [0002] With the development of the liquor industry, the utilization of distiller's grains, a by-product of liquor production, has become the focus of the industry. Solid distiller's grains are the main by-products of liquor production and the main pollutants in liquor production. Because raw materials such as sorghum and wheat are generally used, distiller's grains have high acidity and are rich in protein, amino acids, vitamins and various trace elements. Practice has proved that the degree of comprehensive utilization of distiller's grains directly affects the development of enterprises. If it is not handled properly, the pressure on the environment will be huge; and the level of distiller's grains processed feed is related to the implementation of national feed poli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/12C12R1/11
CPCC12N1/20A23K10/12
Inventor 彭楠常章兵田建平梁运祥
Owner 四川润格生物科技有限公司
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