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Application of adp-ribosylation factor 6 in the prevention and treatment of enterovirus 71 infection

A technology of ribosylation, enterovirus, applied in the field of biomedicine

Active Publication Date: 2018-11-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report on the role of ARF6 molecule in EV71 infection of HBMEC. In-depth research on this molecule can not only improve the understanding of EV71 infection and pathogenic mechanism, but also provide new ideas and targets for the prevention and treatment of EV71 infection

Method used

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  • Application of adp-ribosylation factor 6 in the prevention and treatment of enterovirus 71 infection
  • Application of adp-ribosylation factor 6 in the prevention and treatment of enterovirus 71 infection
  • Application of adp-ribosylation factor 6 in the prevention and treatment of enterovirus 71 infection

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Embodiment 1

[0047] 1 Design and synthesis of specific siRNA sequences for each host cell molecule.

[0048] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis of each target gene, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each gene, a...

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Abstract

The invention relates to the biomedical technology field, and provides a new target and application for enterovirus 71 infection resistance. Human brain microvescular endothelia cell (HBMEC) are employed as target cells, the RNA interference technology is employed to reduce expression of target cell host protein to seek host factors which can inhibit infection of EV 71 to human brain microvescular endothelia cell (HBMEC) effectively, therefore the blood cerebral barrier function is protected, and viruses are prevented from going through blood cerebral barrier to infect the central nervous system. Experiments show that ADP ribosylation factor 6 (ARF6) plays an important role in infection of EV71 to HBMEC, expression of ARF6 is reduced, and infection of EV71 can be inhibited obviously. An application of ARF6 in preparation of medicines preventing or treating enterovirus 71 infection is provided.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and is a new target and application for resisting enterovirus 71 infection. Background technique [0002] Enterovirus 71 (EV71) belongs to the Picornaviridae Enterovirus genus Human Enterovirus A, and is one of the main pathogens that cause Hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand, foot and mouth disease in several major provinces and cities in 2008, the number of people infected and the death rate of the disease have remained high, with more than 1 million cases and nearly 1,000 deaths reported every year. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms on the hands, feet, buttocks and oral mucosa; a small number of children can d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/7088A61P31/14
Inventor 朱勇喆徐庆强戚中田赵平陈生林
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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