Application of agmatine in preparing medicine for treating vascular endothelial injury with syndrome of sepsis
A vascular endothelium and sepsis technology, applied in the field of medicine, can solve the problems of large toxic and side effects, inaccurate drug efficacy, and high price.
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Embodiment 1
[0026] Embodiment 1 Experimental method
[0027] Mice were divided into sham operation group, CLP group, agmatine low-dose group, agmatine middle-dose group, agmatine high-dose group by random number table method, 14 in each group (8 for serum or plasma For the determination of indicators, 6 are only used for the detection of lung and liver vascular permeability). Fasting for 12 hours before operation, free drinking water. Anesthetized by intraperitoneal injection of 1% sodium pentobarbital (1ml / 100g body weight). After anesthesia, it was fixed and routinely disinfected. A 1 cm incision was made along the midline of the abdomen, and the cecum was separated with blunt dissecting forceps and pulled out from the abdominal cavity (to avoid damage to the mesenteric vessels). In the sham operation group, the cecum was brought back into the abdominal cavity and sutured layer by layer; in the CLP group, a No. 3 surgical thread was used to ligate 1 cm from the end of the cecum, and ...
Embodiment 2
[0035] Embodiment 2 experimental result
[0036]1. Expression of serum adhesion molecules ICAM-1 and VCAM-1 (Table 1): 24 hours after modeling, the levels of ICAM-1, VCAM-1 and MCP-1 in the serum of mice in the model group were significantly higher than those in the sham operation group, the difference was statistically significant (P<0.01); the levels of serum ICAM-1, VCAM-1, and MCP-1 in the high-dose agmatine group were significantly lower than those in the sepsis model group, and the difference was statistically significant ( P<0.01) or (P<0.05), the levels of serum ICAM-1, VCAM-1 and MCP-1 in the low-dose agmatine group and the middle-dose group were also significantly lower than those in the sepsis model group, but the effects Intensity is not as high as the high-dose group (results omitted).
[0037] Table 1 Effect of agmatine on serum ICAM-1 and VCAM-1 expression in septic mice
[0038]
[0039] Note: ICAM-1: intercellular adhesion molecule-1, VCAM-1: intervascula...
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