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Plant endosperm-specific strong expression promoter penp4 and its application

A promoter and endosperm technology, which is applied in the field of biotechnology and plant genetic engineering, can solve the problems such as difficult to meet the needs and the lack of endosperm-specific promoters, and achieve improved characteristics, improved reproductive and output characteristics, and improved reproduction and output The effect of the characteristic

Active Publication Date: 2019-08-13
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, there are very few endosperm-specific promoters that have been publicly reported and can be used in research and development and production, and it is difficult to meet the needs

Method used

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  • Plant endosperm-specific strong expression promoter penp4 and its application
  • Plant endosperm-specific strong expression promoter penp4 and its application
  • Plant endosperm-specific strong expression promoter penp4 and its application

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Embodiment Construction

[0045] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0046] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0047] Obtaining the pENP4 Promoter Containing Restriction Sites

[0048] Step 1. Design of primers

[0049] According to the whole genome sequence of the rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice pENP4 gene, and the enzyme cutting sites of the primers were designed according to the characteristics of the selected vector and target gene.

[00...

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Abstract

The invention relates to an endosperm-specific strong expression promoter pENP4 and its application. The present invention isolates a promoter capable of specific and strong expression in plant endosperm. The promoter can guide the specific high expression of the target gene in the plant endosperm, while the low expression or no expression in other tissues of the plant. The invention also provides an expression cassette containing the promoter, a plant expression vector, and a method for obtaining corresponding host bacteria and transformants and applying them to plant genetic engineering. The promoter has important theoretical and practical significance for the research on rice endosperm. The invention will have broad application and market prospects in the field of agriculture, and can be used in conjunction with genes that have improved functions on the endosperm to improve various properties of the rice endosperm.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a rice endosperm-specific expression promoter pENP4 and its application. The promoter can drive the expression of a target gene in a rice transgenic regulation system. Background technique [0002] Rice is one of the most important food crops, and more than one-third of the world's population uses rice as a staple food. With the development of the economy, there are higher and higher requirements on the yield and quality of rice worldwide. Therefore, it is of great significance to use various methods to increase the yield per unit area of ​​rice and improve the quality of rice. Applying modern biotechnology to rice variety improvement can solve some problems that are difficult to solve by conventional methods. Since the 1990s, rice biotechnology has made great progress. The successive isolation of some genes co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/84A01H5/00A01H6/46
Inventor 秦瑞英杨剑波李莉李浩魏鹏程许蓉芳马卉
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI