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Preparation method of phosphatidylserine

A technology of phosphatidylserine and serine, applied in the direction of fermentation, etc., can solve problems such as difficulty in guarantee, large investment in equipment, purity of organic solvent residual products, etc., and achieve the effect of overcoming the complexity of the reaction process

Active Publication Date: 2016-02-03
SINPHAR TIAN LI PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a method for preparing phosphatidylserine from natural lecithin through enzyme-catalyzed reaction. The method is characterized by simplicity and economy. More than 60wt%, so as to overcome the problems of complex reaction process, large equipment investment, high cost, low reaction conversion rate, organic solvent residue and difficult guarantee of product purity existing in the prior art

Method used

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  • Preparation method of phosphatidylserine
  • Preparation method of phosphatidylserine
  • Preparation method of phosphatidylserine

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Weigh 80g of sodium acetate and dissolve it in 1L of water, add acetic acid to adjust the pH value to 5.5; then weigh 5.5g of CaCl 2 Dissolve in 250mL water, finally stir in 1L acetate buffer, 250mL calcium chloride solution, 120g soybean lecithin (phosphatidylcholine content 62.35wt%), 500gL-serine and 3L phospholipase D enzyme solution, heating temperature is 45 ℃, after reacting for 24 hours, filter, and wash the filter residue twice with 1.6L ethanol solution with a concentration of 95% by volume. The detected content was 72.01wt%, and the yield was 62.56wt%.

Embodiment 2

[0025] Weigh 80g of sodium acetate and dissolve it in 1L of water, add acetic acid to adjust the pH value to 5.5; then weigh 5.5g of CaCl 2 Dissolve in 250mL water, finally stir in 1L acetate buffer, 250mL calcium chloride solution, 120g soybean lecithin (phosphatidylcholine content 78.22wt%), 500gL-serine and 3L phospholipase D enzyme solution, heating temperature is 45 ℃, after reacting for 24 hours, filter, and wash the filter residue twice with 1.6L ethanol solution with a concentration of 95% by volume. The detected content was 76.02wt%, and the yield was 68.44wt%.

Embodiment 3

[0027] Weigh 80g of sodium acetate and dissolve it in 1L of water, add acetic acid to adjust the pH value to 5.5; then weigh 5.5g of CaCl 2 Dissolve in 250mL water, finally stir in 1L acetate buffer, 250mL calcium chloride solution, 120g soybean lecithin (phosphatidylcholine content 88.51wt%), 500gL-serine and 3L phospholipase D enzyme solution, heating temperature is 45 ℃, after reacting for 24 hours, filter, and wash the filter residue twice with 1.6L ethanol solution with a concentration of 95% by volume. The detected content was 79.83wt%, and the yield was 74.31wt%.

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Abstract

The invention relates to a preparation method of phosphatidylserine. The preparation method comprises the steps of carrying out enzymic catalytic reaction on natural lecithin and L-serine in the presence of phospholipase D enzyme liquid, and carrying out a simple purification process after the reaction, so as to obtain phosphatidylserine with the yield exceeding 60%. The preparation method has the beneficial effects that the operation is simple and convenient, the yield is high, the cost is low, the product quality is stable, and the process is environmentally friendly.

Description

technical field [0001] The invention relates to a preparation method of a compound, in particular to a method for preparing phosphatidylserine from natural lecithin through an enzyme-catalyzed reaction. Background technique [0002] Phosphatidylserine (phosphatidylserine), also known as serine phospholipid, is a kind of ubiquitous phospholipid, usually located in the inner layer of cell membrane, and phosphoglycerides in phospholipid compounds are one of the components of cell membrane. Associated with a range of membrane functions. Especially in the nervous system of the human body, it is one of the important components of the cell membrane of the brain. At the same time, it plays an important role in regulating various functions of the brain (especially the memory and emotional stability of the brain). fluidity, permeability, and can activate the metabolism and synthesis of various enzymes. It has important functions and functions: delaying aging, maintaining a healthy a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/06
Inventor 王昭日杨胜杰刘明川黃佳慧杨进平刘敏唐静静
Owner SINPHAR TIAN LI PHARMA
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