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A molecular marker related to early weight gain of Gaoyou ducks, its acquisition method and application

A molecular marker, Gaoyou duck technology, applied in the field of poultry genetic engineering, can solve the problems that have not yet been discovered, and achieve the effect of low cost

Inactive Publication Date: 2019-06-21
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecular markers and applications related to the early weight gain of Gaoyou ducks have not been found in the prior art

Method used

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  • A molecular marker related to early weight gain of Gaoyou ducks, its acquisition method and application
  • A molecular marker related to early weight gain of Gaoyou ducks, its acquisition method and application
  • A molecular marker related to early weight gain of Gaoyou ducks, its acquisition method and application

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Embodiment Construction

[0013] The present invention will be explained in detail below in conjunction with the embodiments.

[0014] The molecular marker related to the early weight gain of Gaoyou ducks of the present invention is located at the nucleotide at the 2619 site of exon 2 of the GH gene, and the nucleotide at this site is A or G, resulting in the The single nucleotide polymorphism of the point, because the GH gene sequence is too long, the partial sequence of the gene is intercepted and listed. The nucleotide sequence is shown in SEQ ID NO:1.

[0015] Aiming at the SNP polymorphism at the 2619 site of exon 2 of the above-mentioned GH gene, the present invention also discloses its acquisition method, wherein the pair of forward and reverse primers used to amplify the GH gene and detect the mutation of the gene site The sequences are shown in SEQ ID NO: 2-3.

[0016] Wherein it is used to perform ligase detection reaction (LDR) on the above amplification products, and the required LDR prob...

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Abstract

The invention discloses a molecular marker related to early weight gains of Gaoyou ducks as well as an obtaining method and an application thereof. The molecular marker is located in nucleotide at the 2619th site of a second exon of a GH (growth hormone) gene, wherein the nucleotide at the site is A or G; and a nucleotide sequence of the GH gene is shown in SEQ ID NO:1. The obtaining method is characterized by carrying out PCR (polymerase chain reaction) amplification on Gaoyou duck genome DNA (deoxyribonucleic acid), sequencing an amplification product after carrying out ligase detection reaction on the amplification product and obtaining the molecular marker related to early weight gains of Gaoyou ducks through sequence analysis. The molecular marker is applied to screening characters related to early weight gains of Gaoyou ducks, can be used in early selection in family selection and can provide molecular basis in apolegamy. The molecular marker provides a new method for screening the characters related to early weight gains of Gaoyou ducks.

Description

technical field [0001] The invention belongs to the technical field of poultry genetic engineering, and more specifically relates to a molecular marker related to early weight gain of Gaoyou ducks, an acquisition method and application thereof. Background technique [0002] Growth Hormone (GH) is a single peptide chain protein hormone synthesized and secreted by eosinophils in the anterior pituitary gland. The main effects of GH on the animal body are that it can significantly increase the growth rate, promote muscle growth, and reduce fat content. In poultry, during the embryonic period, injection of exogenous GH can significantly improve the quality of chicken embryos, promote the development of long bones and cartilage tissues, and lead to an increase in market weight of broiler commercial chickens. Studies on ducks have found that the distribution of GH genotype frequencies is related to breeds; SNP sites in GH gene coding regions and regulatory regions are closely rela...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李慧芳徐文娟朱春红宋卫涛陶志云刘宏祥章双杰王晓峰张静
Owner JIANGSU INST OF POULTRY SCI