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Cell plate clone formation image acquisition instrument

An image acquisition and flat-panel technology, which is applied in the field of image acquisition instruments, can solve problems such as poor image quality, inappropriate image acquisition angle, and impact on result analysis, and achieve the effect of uniform light and favorable image capture

Inactive Publication Date: 2016-02-03
FIRST HOSPITAL OF SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention mainly aims at the problems of inappropriate image acquisition angle, poor image quality and affecting result analysis in the way of artificially using a digital camera to take pictures after cell clone formation, and provides an image acquisition instrument for cell plate clone formation

Method used

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  • Cell plate clone formation image acquisition instrument
  • Cell plate clone formation image acquisition instrument

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Cell clone formation experiments:

[0020] 48 hours after cell transfection or drug administration, the cells of different groups were collected by trypsinization, and the cells were resuspended in 1 mL of medium and counted. Pipette into a single cell suspension, count the cells, and dilute the cells to 1×10 4 cells / ml, then diluted to 1×10 3 cells / ml inoculated in multi-well plates or petri dishes, inoculate with 50uL at 50cell / well, inoculate with 100uL at 100cell / well, inoculate with 200uL at 200cell / well. After inoculation, add culture medium to each well to 300uL / well. Shake the culture dish or multi-well plate horizontally back and forth to make the cells in the wells evenly distributed. After culturing for 7 days, suck off the medium in each well, rinse twice with PBS or normal saline, add 200uL crystal violet staining solution to each well to fully cover the bottom of the well, and wash the multi-well plate or culture dish in PBS buffer after 20 minutes. Th...

Embodiment 2

[0022] A kind of cell plate colony formation image acquisition instrument, such as figure 1 and figure 2 As shown, it includes a collection bin 1, wherein: the collection bin 1 is a cube structure with an open front and bottom, and the four columns 7 of the collection bin 1 are telescopic rod structures, and the bottom of the collection bin 1 is provided with A light board 2 is provided with a cold light source LED lamp 3 with a color temperature of natural light above the light board 2, and a plurality of lamps 3 are arranged in a "back" shape. The light source backboard 4 made of light acrylic board is provided with a lens hole 5 on the top plate of the collection bin 1. The lens hole 5 is a circular hole with a diameter of 10 cm. The sample placement plate 6 made of the plate, and there are two sample placement plates 6, and two parallel rows of petri dish grooves 8 are opened on one sample placement plate 6, with 3 in each row, two rows aligned up and down and equally sp...

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Abstract

The invention relates to an image acquisition instrument with lighting and image acquisition functions, and specifically relates to an image acquisition instrument for image acquisition after a cell plate clone experiment for research of cytobiological function and tumor cell function. The image acquisition instrument mainly solves the problems that if only a digital camera is used by manpower to take pictures after cell clone formation, the image acquisition angle is unsuitable, and the image quality is low and the result analysis is influenced. The cell plate clone formation image acquisition instrument includes an acquisition house, wherein one part of the bottom and one part of the side wall of the acquisition house are open-mouthed structures; a light panel is arranged at the bottom of the acquisition house; a plurality of lights are arranged above the light panel; a light source backboard is arranged above the lights and at the middle lower part of the acquisition house; a lens hole is formed in a top board of the acquisition house; and a sample placing plate is movably arranged on the light source backboard.

Description

technical field [0001] The invention relates to an image acquisition instrument with lighting and image acquisition functions, and specifically relates to an image acquisition device for collecting cells after plate cloning experiments in the research of cell biology functions and tumor cell functions. Background technique [0002] The cell plate cloning experiment reflects the number of cells that adhere to the wall and form clones after inoculation. Not every adherent cell can proliferate and form clones, but the cells that form clones must be adherent and proliferative cells. Clonogenicity can reflect two important traits of cell population dependence and proliferation ability, and is a classic experiment for evaluating the malignant biological characteristics of cells, especially tumor cells. However, there is no suitable equipment for image collection after plate cloning. The traditional method only uses digital cameras to take pictures manually. This method has the fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G03B15/05G03B15/06G03B15/03
CPCG03B15/05G03B15/06
Inventor 高伟吴勇延张春明张哲王斌全
Owner FIRST HOSPITAL OF SHANXI MEDICAL UNIV