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Weeping oriental cherry tissue culture method

A technology of tissue culture and cherry blossoms, which is applied in the field of plant tissue culture, can solve the problems of shortened internodes and low proliferation coefficient, and achieve low pollution rate and good start-up effect

Active Publication Date: 2016-02-17
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of this invention is to provide a kind of tissue culture method of weeping cherry, to overcome the internode shortening that exists in the tissue culture of existing weeping cherry, the problem of low multiplication coefficient

Method used

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  • Weeping oriental cherry tissue culture method
  • Weeping oriental cherry tissue culture method
  • Weeping oriental cherry tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Material: The material is "Weeping Cherry Blossom", one of the varieties of cherry blossoms, and the stems with axillary buds that were born in the current year are selected.

[0030] The tissue culture method of the weeping cherry of the present embodiment comprises the following steps:

[0031] 1. Material collection

[0032] At noon in fine weather conditions from March to May, the middle and upper parts of healthy and disease-free shoots of weeping cherry blossoms were intercepted as explants, and the leaves were removed to leave petioles. When collecting explants, pay attention to the quality, purity and accuracy of the selected materials, and do not take them casually to avoid unnecessary losses. Select spring branches that grow robustly and are free from diseases and insect pests as materials, and inoculate the collected materials in time to prevent wilting due to water loss or pollution and mildew.

[0033] 2. Material pretreatment

[0034] Trim the collected...

Embodiment 2

[0047] The optimization of embodiment 2 proliferation medium formula

[0048] Except that the formulation of the proliferation medium is different from that of Example 1, the rest of the method steps are the same as that of Example 1.

[0049] 1. Effects of different concentrations of 6-BA and NAA on the proliferation of weeping cherry blossoms

[0050] The formula of the proliferation medium is: MS+6-BA+NAA+sucrose 30g / L+agar 6.5g / L, and the pH value is adjusted to 5.8. See Table 1 for the concentrations of 6-BA and NAA.

[0051] Table 16-The effect of different concentrations of BA and NAA on the proliferation of weeping cherry blossoms in tissue culture

[0052]

[0053] As shown in Table 1, the proliferation coefficients of all treatments were low, between 1.1 and 2.2.

[0054] 2. Effects of basal medium, 6-BA and NAA on the proliferation of weeping cherry

[0055] The formula of the proliferation medium is: basic medium+6-BA+NAA+sucrose 30g / L+agar 6.5g / L, the pH value...

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Abstract

The invention discloses a weeping oriental cherry tissue culture method including the steps: preparation of an explant, initiation culture, multiplication culture and rooting culture; a culture medium of the multiplication culture contains 0.2-0.5 mg / L of 6-BA, 0.03-0.07 mg / L of NAA and 5-15 mg / L of GA. The weeping oriental cherry tissue culture method has the advantages of lower contamination rate and good starting effect; the induced differentiation rate of the initiation culture can reach 84%; in the multiplication culture stage, weeping oriental cherry can perform normal jointing, tissue-cultured seedlings grow healthy and strong, the problems of shortened internode and easy aging generally appearing in a traditional oriental cherry tissue culture process are solved, and the multiplication coefficient can reach 7.0.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of weeping cherry blossoms. Background technique [0002] The traditional propagation method of cherry blossoms is grafting, but it is slow to grow into seedlings and it is difficult to mass-produce quickly. With the development of tissue culture technology, tissue culture provides an effective way for the rapid propagation and factory production of cherry blossoms. Domestic scholars' research on cherry blossoms mainly focuses on the types and varieties of cherry blossoms such as mountain cherry, Tokyo cherry, Fujian mountain cherry, and wild cherry. Weeping cherry blossoms are ornamental cherry blossoms that have only been developed in recent years. Because of their soft and elegant branches and beautiful appearance, they are more and more used in landscaping and landscaping. [0003] The research on the tissue culture of weeping cherry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 王红梅李园莉胡卫霞刘春风于健
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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