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Application of raltitrexed or pharmaceutically-acceptable raltitrexed salt to preparation of drugs for treating or preventing herpesvirus infection

A technology of herpes virus infection, raltitrexed, applied in the field of biomedicine to achieve a good effect of anti-herpes virus activity

Inactive Publication Date: 2016-02-17
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, drugs to treat or prevent herpes virus infection still need to be further developed

Method used

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  • Application of raltitrexed or pharmaceutically-acceptable raltitrexed salt to preparation of drugs for treating or preventing herpesvirus infection
  • Application of raltitrexed or pharmaceutically-acceptable raltitrexed salt to preparation of drugs for treating or preventing herpesvirus infection
  • Application of raltitrexed or pharmaceutically-acceptable raltitrexed salt to preparation of drugs for treating or preventing herpesvirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Detection of the activity of raltitrexed in inhibiting KSHV lytic phase replication

[0034] 1. Experimental materials

[0035] 1.1 Cells and viruses

[0036] The iSLK.219 cell was donated by Professor Dr. DonGanem. The cell is a SLK cell containing the recombinant virus rKSHV.219 and the RTA expression system regulated by Doxycycline. In this cell, the RTA expression sequence was constructed into the pRetro-XTet-ON inducible expression system (Clontech, Mountainview, CA), and the expression of this system was regulated by doxycycline. In the presence of doxycycline, RTA Expression can occur; Vero cells were preserved in our laboratory and purchased from American Type Culture Collection (ATCC).

[0037] Raltitrexed was purchased from Biochempartner.

[0038] 1.2 Reagents

[0039] DMEM medium, 1640 medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; SYBR mixture (iTaq TM Universal GreenSupermix) was purchased from ...

Embodiment 2

[0065] Embodiment 2: the detection of raltitrexed anti-HSV-1 and HSV-2 activity

[0066] 1. Experimental materials

[0067] 1.1 Cells and viruses

[0068] Vero cells were preserved in our laboratory and purchased from the American Type Culture Collection (ATCC). HSV-1 is an F strain, which is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences. HSV-2 is a G strain, which is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences.

[0069] 1.2 Reagents

[0070] DMEM medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega.

[0071] 1.3 Experimental Instruments

[0072] The multi-label microplate reader and the high-content cell analyzer were purchased from PerkinElmer; the cell incubator was a product of Thermo.

[0073] 2. Experimental methods and results

[0074] 2.1 Cytotoxicity detection of raltitrexed on Vero cells

[0075] (1) Divide Vero cells into 8×10 3 Each cell / well was see...

Embodiment 3

[0094] Embodiment 3: Raltitrexed inhibits the detection of EBV lytic phase replication

[0095] 1. Experimental materials

[0096] 1.1 Cells and viruses

[0097] The B95-8 cell was donated by Professor Sun Xiaoping of the School of Basic Medicine of Wuhan University. The cell contains the complete sequence of the EBV virus genome. Combined induction with 20ng / mLTPA can make the virus enter the lytic phase and replicate.

[0098] 1.2 Reagents

[0099] 1640 medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; SYBR mixture (iTaq TM Universal GreenSupermix) was purchased from Bio-Rad Company.

[0100] 1.3 Experimental Instruments

[0101] The multi-marker microplate reader was purchased from PerkinElmer; the fluorescent quantitative PCR instrument (Bio-RadCFX96Touch TM Real-Time PCR detection system) was purchased from Bo-Rad Company; Type refrigerated centrifuge and cell culture box were purchased from Thermo Company. ...

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Abstract

The invention discloses application of raltitrexed or pharmaceutically-acceptable raltitrexed salt to preparation of drugs for treating or preventing herpesvirus infection. Raltitrexed totally without toxin concentration is selected and used for performing an anti-herpesvirus test, a result shows that the compound has remarkable anti-herpesvirus activity and is in dose-dependent correlation, and raltitrexed inhibits virus copying to display the anti-herpesvirus effect. Detection on different subtypes of herpesviruses displays that raltitrexed has wide anti-herpesvirus activity, the selection index is high, raltitrexed can be developed into drugs for treating herpesvirus infection, and wide application prospects are achieved.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular, the invention relates to the application of raltitrexed or a pharmaceutically acceptable salt thereof in the preparation of medicines for treating or preventing herpes virus infection. Background technique [0002] Herpesviridae is a family of viruses with envelopes and double-stranded DNA genomes. Members of this family can widely infect animals and humans and induce corresponding diseases. There are currently eight herpes viruses that can infect humans, including: Herpes simplex virus-1 (HSV-1), Herpes simplex virus-2 (HSV-2), Varicella-Zoster Herpes virus (Varicellazostervirus, VZV), Epstein-Barrvirus (EBV), cytomegalovirus (Cytomegalovirus, CMV), human herpesvirus type VI (HHV-6), human herpesvirus type VII (HHV-7) and card Bordeaux herpes virus (Kaposi's sarcoma-associated herpesvirus, KSHV). According to the differences in genome sequence, structure and physical and chemical pro...

Claims

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Application Information

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IPC IPC(8): A61K31/4709A61P31/22
Inventor 陈绪林陈军刚
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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