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A SNP Molecular Marker Tightly Linked to Sesame Seed Coat Color Gene

A molecular marker and sesame technology, applied in the field of molecular genetics and breeding, can solve problems such as research that needs to be deepened, achieve reliable results, stable detection results, and improve breeding efficiency

Active Publication Date: 2018-01-12
HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, due to the variety of seed coat colors in sesame seeds, the research on traits is easily affected by the development and maturity of seeds, and the research on the color genes of sesame seed coats still needs to be further studied.

Method used

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  • A SNP Molecular Marker Tightly Linked to Sesame Seed Coat Color Gene
  • A SNP Molecular Marker Tightly Linked to Sesame Seed Coat Color Gene
  • A SNP Molecular Marker Tightly Linked to Sesame Seed Coat Color Gene

Examples

Experimental program
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Effect test

Embodiment 1

[0050] This example briefly introduces the SNP molecular markers closely linked to the seed coat color gene of sesame seeds Si The screening process of SCC2385, the specific process is as follows.

[0051] 1. Construction of black and white sesame F2 genetic populations

[0052] In July 2013, the white sesame line Yuzhi DS899 and the black sesame germplasm JS012 were used to configure a hybrid combination to obtain F1.

[0053] In November 2013, the F1 seeds were sown on-demand in a nutrient bowl and planted in the Sanya Base of the Sesame Research Center of the Henan Academy of Agricultural Sciences. After 2 pairs of true leaves, each plant was transplanted in time to ensure that the number of lines was greater than 300.

[0054] 2. SNP genetic map construction and QTL mapping of F2 population

[0055] (1) Genome resequencing of F2 population parents and 122 F2 individual plants

[0056] 120 lines were randomly selected from the F2 population, and the young leaves of 120 l...

Embodiment 2

[0083] This embodiment mainly introduces the SNP molecular marker closely linked with the sesame seed coat color gene Si The verification of SCC2385, that is, the detection method of the molecular marker, the specific process is as follows.

[0084] (1) Extract the genomic DNA of the sesame germplasm resources to be tested

[0085] This embodiment is based on the reconstructed testa coat color-related F2 population, and the SNP molecular marker Si SCC2385 has been detected, and the construction process of the F2 population is as follows:

[0086] In July 2014, the white sesame line Yuzhi DS899 and the black sesame germplasm JS012 were used to form a hybrid combination at the Yuanyang Base of the Sesame Research Center of the Henan Academy of Agricultural Sciences, and F1 was obtained;

[0087] In November 2014, F1 generation seeds were planted in the Sanya base, and a single plant was covered with nets for self-crossing, and then F2 generation seeds were obtained, with a pop...

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Abstract

The invention belongs to the technical field of molecular genetic breeding, and in particular relates to a SNP molecular marker SiSCC2385 closely linked with a seed coat color gene of sesame seeds. The marker is located in the 9th linkage group in the sesame SNP genetic map, 195.48‑197.79cM, and the interpretation rate of the seed coat color is 87.5%~90% (Vg / Vp). The marker contains 103bp, and its sequence is shown as SEQ ID No. 1. The invention innovates the sesame breeding technology. Compared with the currently used conventional breeding technology method, the detection method is simple and efficient, and the result is reliable; the provided molecular marker SiSCC2385 can significantly improve the breeding efficiency and save scientific research costs when used in sesame molecular assisted breeding and time, and lay a solid foundation for improving the sesame molecular breeding technology platform.

Description

technical field [0001] The invention belongs to the technical field of molecular genetic breeding, and in particular relates to a SNP molecular marker closely linked with the seed coat color gene of sesame seeds Si SCC2385. Background technique [0002] Sesame( Sesamum indicum L., 2n=26), belongs to the genus Linaceae, and is one of the oldest characteristic high-quality oil crops in the world. my country is one of the four major producers of sesame, with an annual planting area of ​​about 12 million mu and a total output of about 750,000 tons. According to the color of the seed coat, sesame is divided into white sesame and black sesame. In my country, the main producing areas of white sesame are mainly distributed in Henan, Anhui and Hubei provinces, and the main producing areas of black sesame are mainly located in Jiangxi Province. Due to the pure color, excellent appearance and internal quality, the production of white sesame and black sesame in China enjoys a high ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 苗红梅张海洋李春魏利斌段迎辉徐芳芳张体德常淑娴曲文文
Owner HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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