Gene rEg.myophilin molecular engineering vaccine resistant to sheep echinococcosis infection and preparation method and application thereof

A hydatid disease, genetic technology, applied in the field of genetic engineering

Inactive Publication Date: 2016-02-24
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide the application of a gene molecule rEg.myophilin in the preparation of a vaccine ag

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  • Gene rEg.myophilin molecular engineering vaccine resistant to sheep echinococcosis infection and preparation method and application thereof
  • Gene rEg.myophilin molecular engineering vaccine resistant to sheep echinococcosis infection and preparation method and application thereof
  • Gene rEg.myophilin molecular engineering vaccine resistant to sheep echinococcosis infection and preparation method and application thereof

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Embodiment Construction

[0016] Vaccine preparation:

[0017] A method for preparing a gene rEg.myophilin molecular engineering vaccine against sheep echinococcosis infection. First, extract RNA from the Chinese strain of Echinococcus granulosus and obtain the Eg.myophilin gene by RT-PCR. The gene is included in the GenBank database, sequence No.: DQ679473, Eg.myophilin was subcloned into the expression vector pET-28a, then transformed into Escherichia coli BL21(DE3)plysS to induce expression, and then purified the protein with the His-bind resin purification kit containing Ni2+ from Novagen;

[0018] In order to confirm the immune protection of recombinant antigen vaccine rEg.myophilin against echinococcosis infection, the present invention is based on the natural infection of echinococcosis eggs in sheep, and prevents rEg.myophilin through primary immunization-boost immunization (prime-boost), Molecular biology, cell biology, immunology and other methods were used to evaluate the protection of the v...

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Abstract

The invention relates to a gene rEg.myophilin molecular engineering vaccine resistant to sheep echinococcosis infection. The vaccine is obtained by extracting RNA from a China strain of Echinococcus granulosus, obtaining Eg.myophilin gene through RT-PCR and performing colonizing, induced expression and purification, and collected under CCTCC M2015426. The invention further relates to application of rEg.myophilin gene molecules in preparing vaccines resistant to sheep echinococcosis infection. Immunoprotection verification of an Echinococcus granulosus recombinant vaccine is performed on sheep, and immune protection efficiency reaches 92%. The vaccine is expected to play a great role in preventing and treating liver echinococcosis.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a gene rEg.myophilin molecular engineering vaccine against sheep echinococcosis infection, its preparation method and application technology Background technique [0002] Echinococcus granulosus, commonly known as echinococcosis, currently has no commercially available vaccine. At present, there are more than ten kinds of candidate molecules, such as Eg95, rEg14-3-3 recombinant vaccine, ferritin recombinant vaccine, GST recombinant vaccine, etc. Among them, EG95 was first confirmed to be a sheep protective Echinococcus granulosus vaccine. The protective power of EG95 is 96-98%. Among them, domestic Kang Qiang and others have also verified the protective power of EG95 on sheep, and obtained a protective power of 90%. It is a pity that Eg95 was first successfully developed by foreign countries. Even if it is developed into a real product, its intellectual property rights...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K48/00A61P33/10C12N15/12C12N15/70C07K14/435
Inventor 赵巍
Owner NINGXIA MEDICAL UNIV
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