Method for producing graft material for treating nerve damage

A transplantation material and nerve injury technology, which is applied in the field of manufacturing transplantation materials for nerve injury treatment, can solve the problems of undeveloped fundamental methods for treatment and difficulty in functional reconstruction, and achieve the effect of high motor function and high recovery effect.

Inactive Publication Date: 2016-03-02
ADVANCED CELL TECH & ENG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not only is it an effective treatment, but even partial functional reconstruction is very difficult
In recent years, its research has been actively carried out in the world, but the fundamental method of treatment has not yet been developed

Method used

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  • Method for producing graft material for treating nerve damage
  • Method for producing graft material for treating nerve damage
  • Method for producing graft material for treating nerve damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0164] Example 1. The movement of total spinal cord resection model caused by different culture methods of dental pulp stem cells Influence of functional restoration effect 1

[0165] 1. Experimental method

[0166] 1-1. Animals and materials

[0167] Wistar rats (7 weeks old, female) were purchased from Japan SLC Company, and anesthetic pentobarbital (somnopentyl) was purchased from Kyoritsu Pharmaceutical Co., Ltd. Before carrying out animal experiments, based on the relevant regulations on the safety and welfare of animal experiments, an animal experiment plan was formulated according to the prescribed format, which was approved by the Animal Breeding and Animal Experiment Committee of Gifu Pharmaceutical University.

[0168] 1-2. Cell culture

[0169] The method of inducing, culturing and proliferating dental pulp stem cells from extracted teeth was carried out according to the published method (Tamaoki et al., JDentRes. 201089:773-778). The following cells were pre...

Embodiment 2

[0177] Example 2. Movement of total spinal cord resection model caused by different culture methods of dental pulp stem cells Influence of functional recovery effect 2

[0178] 1. Experimental method

[0179] 1-1. Animals and materials

[0180] Experimental animals were prepared in the same manner as in Example 1.

[0181] 1-2. Cell culture

[0182] The method of inducing, culturing and proliferating dental pulp stem cells from extracted teeth was carried out according to the published method (Tamaoki et al., JDentRes. 201089:773-778). The following cells were prepared: Dental pulp stem cells subcultured to passage 12 with MSCBM medium (LONZA), and dental pulp after subcultured 7-8 times with α-MEM medium (Sigma Company) containing 10% FCS Stem cells (DP31S); and dental pulp stem cells (DP31F) subcultured 7-8 times with α-MEM medium containing 10 ng / ml FGF2 and 10% FCS.

[0183] 1-3. Experimental method using full-cut model

[0184] The same total spinal cord resectio...

Embodiment 3

[0189] Example 3. The total spinal cord resection model caused by the difference in donors of dental pulp stem cells Influence of motor function recovery effect

[0190] 1. Experimental method

[0191] 1-1. Animals and materials

[0192] Experimental animals were prepared in the same manner as in Example 1.

[0193] 1-2. Cell culture

[0194] Dental pulp stem cells were induced from teeth extracted from three different donors (DP31, DP74, and DP264), cultured and proliferated according to a published method (Tamaoki et al., JDentRes. 201089:773-778) . The following cells were prepared: dental pulp stem cells (DP31F, DP74F and DP264F) from 3 different donors subcultured 7-8 times with α-MEM medium containing 10 ng / ml of FGF2 and 10% FCS.

[0195] 1-3. Experimental method using full-cut model

[0196] The same total spinal cord resection model as in Example 1 was prepared.

[0197] 2. Results

[0198] Same as Example 1, the recovery of motor function is evaluated by B...

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Abstract

The present invention addresses the problem of providing a method for producing a graft material, which has a high restoring effect on dysfunction caused by nerve damage, at a high efficiency and a high reproducibility. Provided are a method for producing a graft material for treating nerve damage, etc., said method comprising a step for culturing dental pulp stem cells in a medium that is substantially free from growth factors other than FGF2.

Description

technical field [0001] The present invention relates to a method and the like for producing a transplant material for nerve injury treatment using dental pulp stem cells. Background technique [0002] The spinal cord is a pathway that transmits motor and sensory information between peripheral tissues and the brain. If it is damaged, it will cause severe limb impairment such as motor paralysis or sensory impairment. Not only is it an effective treatment, but even partial functional reconstruction is very difficult. In recent years, its research has been actively carried out in the world, but the fundamental method of treatment has not yet been developed. [0003] There are about 100,000 patients with spinal cord injury in my country (Japan), and about 5,000 new injured patients are added every year. It is concentrated around the age of 20 and 60, mainly in the middle-aged, and is caused by injuries caused by accidents during driving or sports, or vertebral fractures caused ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074A61K35/32A61P25/00
CPCA61K35/32C12N5/0664C12N2501/115A61P25/00A61P25/02A61L27/3834A61L27/3878A61L27/3895A61L27/54A61L2300/252A61L2300/414A61L2300/64A61L2430/32
Inventor 手塚建一川口知子国贞隆弘柴田敏之福光秀文古川昭荣
Owner ADVANCED CELL TECH & ENG LTD
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