A statistical method and special statistical instrument for fruit fly fecundity

Abstract

The invention discloses a drosophila fecundity statistical method, which comprises steps of pupa counting and adult drosophila counting. A special pupa counter is used, a bottom silica gel bent shovel is used for sequentially moving the pupas to an eclosion bottle, and the counter carries out counting at the same time until no pupas are formed in a culture bottle; the eclosion bottle is put in a culture box and observed regularly each day, and when an adult drosophila appears, a special adult drosophila counter is used for counting the daily adult drosophila number at the same time each day; all daily pupa numbers and all daily adult drosophila numbers are added, and thus, the pupa number and the adult drosophila number of the drosophilae in the culture bottle are obtained; and the ratio of the two is the adult drosophila rate of the drosophilae. The statistical method of the invention is simple and accurate; three important biological effect indexes which can reflect the fecundity of the drosophila can be calculated accurately; and the special pupa counter and the special adult drosophila counter are simple to operate, the workload of a researcher is reduced, no damage is caused to the drosophila, and the experiment result can be ensured to be accurate.

Description

technical field [0001] The invention relates to genetics and developmental biology research, in particular to a statistical method for the fecundity of fruit flies, and also relates to a special statistical tool for the statistical method—an adult fly counter. Background technique [0002] Drosophila is an important experimental material in genetics and developmental biology research. It is a complete metamorphosis insect, and its life cycle can be divided into four stages: egg, larva, pupa and adult fly. Adult flies lay their eggs on the surface of the culture medium in the culture bottle after fertilization, and the eggs hatch into larvae, which grow up by looking for nutrients in the culture medium. The larvae in the pupa further develop various tissues and organs of the adult fly, and finally emerge from one end of the pupa as a virgin fly. After about eight hours, the male and female flies will mate and produce the next generation. This is the life of a fruit fly. cycl...

AI Technical Summary

Problems solved by technology

Due to the strong fecundity of Drosophila, the number of newly formed pupae every day is very large, and it is very easy to make mistakes when counting, and some pupae will fall off the bottle wall and fall on the medium or knot on the surface of the medium, which is not easy to be found

Adult flies have strong activity and are always in a state of motion. When the number is large, it is very difficult to count. If the existing ether anesthesia method or low temperature anesthesia method is used to count, it is very easy to cause damage to Drosophila and affect the survival of Drosophila. longevity and reproductive capacity

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