Application of C. nigricans Extract in Whitening and Moisturizing Cosmetics
A technology of carbonobacteria nigericum and extract, which is applied in the directions of cosmetics, cosmetic preparations, cosmetic preparations, etc., to achieve the effects of inhibiting activity, improving economic use value, and broadening application fields
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Embodiment 1
[0037] Rinse the sclerotia of C. nigritiformis with sterile water, then sterilize the surface with 75% alcohol by volume, cut into pieces of about 2mm×2mm size on the ultra-clean table, and inoculate them into the improved PDA slant medium (in Add 0.45% of yeast extract and 0.5% of potassium dihydrogen phosphate to the PDA basic medium, and then enter the fermenter for fermentation after conventional cultivation at 25°C to 30°C, and the inoculum size is 10% by volume The fermentation time is about 2-3 days, the fermentation process parameters are pH value 5.0-7.0, stirring speed 120-250rpm, ventilation volume 1-4VVM, fermentation temperature 25℃-30℃, tank pressure 0.03-0.05MPa, The fermentation medium consists of 90g of glucose, 3g of ammonium sulfate, 1g of dipotassium hydrogen phosphate trihydrate, 4g of glutamic acid, 2g of triammonium citrate, 5mg of ferrous sulfate heptahydrate, and 4mg of zinc sulfate per liter, and the pH is adjusted to 5-5.5. After the fermentation is ...
Embodiment 2
[0039] Appropriate amount of mycelia of C. nigritiformis (prepared in Example 1) was pulverized, passed through a 80-mesh sieve, added drinking water equivalent to 30 times the quality of the mycelium, heated to 80°C, and used ultrasonic waves (power of 120W) at this temperature Extract for 90 minutes, filter to obtain the filtrate, concentrate to a density of 1.02, and obtain the extract of C. nigritiformis.
Embodiment 3
[0041] Appropriate amount of Sclerotia nigriscens sclerotium was crushed, passed through 80 mesh sieve, added drinking water equivalent to 20 times the quality of sclerotia, heated to 80°C, extracted with ultrasonic waves (power 120W) for 90 minutes at this temperature, and filtered to obtain The filtrate is concentrated to an appropriate amount, left to cool, slowly adding 95% ethanol by volume in a stirring state, so that the ethanol concentration in the finally obtained mixed solution is 65% by volume, sealed for 12 hours, and the precipitation is complete, and the supernatant is taken, The ethanol was recovered under reduced pressure, concentrated, and then filtered through a 0.45 μm microporous membrane to obtain the extract of C. nigritiformis.
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