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The rapid propagation method of star anise lotus

A technology of star anise and star anise, which is applied in the field of rapid propagation of star anise, can solve the problems that there are no reports of somatic embryos of star anise plants, and achieve the effects of improving disease resistance, high plant regeneration frequency, and fast growth

Active Publication Date: 2018-02-16
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, except for the research results of our laboratory, there is no report about the somatic embryogenesis of the star anise plant

Method used

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  • The rapid propagation method of star anise lotus
  • The rapid propagation method of star anise lotus

Examples

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Effect test

Embodiment 1

[0039] A kind of rapid propagation method of star anise lotus, comprises the following steps:

[0040] Step 1: making sterile explants of star anise lotus, selecting leaves, roots or stems of star anise lotus as explants, placing the explants in a beaker, soaking them with amphoteric surfactants, and placing them in ultrasonic waves Vibrate for 10min, rinse with deionized water for 40min, gently scrub the dirt on the surface of the explant with a soft brush in the rinse process, then blot the moisture outside the explant with sterile filter paper and cut into 5mm 2 To obtain the aseptic explants, the explants of Star Anise are first soaked in an amphoteric surfactant for disinfection. The alkaline amphoteric surfactant of beetroot has good antibacterial properties and can inhibit the growth of some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability for divalent copper, and the diva...

Embodiment 2

[0057] A kind of rapid propagation method of star anise lotus, comprises the following steps:

[0058] Step 1: making sterile explants of star anise lotus, selecting leaves, roots or stems of star anise lotus as explants, placing the explants in a beaker, soaking them with amphoteric surfactants, and placing them in ultrasonic waves Vibrate for 20min, rinse with deionized water for 50min, gently scrub the dirt on the surface of the explant with a soft brush during the rinse, then blot the moisture outside the explant with sterile filter paper and cut into 5mm 2 To obtain the aseptic explants, the explants of Star Anise are first soaked in an amphoteric surfactant for disinfection. The alkaline amphoteric surfactant of beetroot has good antibacterial properties and can inhibit the growth of some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability for divalent copper, and the divalent...

Embodiment 3

[0075] A kind of rapid propagation method of star anise lotus, comprises the following steps:

[0076] Step 1: making sterile explants of star anise lotus, selecting leaves, roots or stems of star anise lotus as explants, placing the explants in a beaker, soaking them with amphoteric surfactants, and placing them in ultrasonic waves Vibrate for 15min, rinse with deionized water for 45min, gently scrub the dirt on the surface of the explant with a soft brush during the rinse, then blot the moisture outside the explant with sterile filter paper and cut into 5mm 2 To obtain the aseptic explants, the explants of Star Anise are first soaked in an amphoteric surfactant for disinfection. The alkaline amphoteric surfactant of beetroot has good antibacterial properties and can inhibit the growth of some bacteria and fungi. Growth and reproduction, at the same time, the alkyl dimethyl betaine in the amphoteric surfactant has a good chelating ability for divalent copper, and the divalent...

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Abstract

The invention discloses a method for rapid propagation of star anise lotus, which comprises the following steps: step 1: making sterile explants of star anise lotus; step 2: cultivating the sterile explants by using induction medium to obtain embryogenic callus Tissue; step 3: using the first proliferation medium to cultivate the embryogenic callus, and subcultured for 2 to 3 generations to obtain the subcultured callus; step 4: using the differentiation medium to cultivate the described subcultured callus to obtain somatic embryos; step 5: cultivating the somatic embryos with a second proliferation medium to obtain secondary somatic embryos; step 6: cultivating the secondary somatic embryos with plant regeneration medium to obtain star anise lotus plants. In the present invention, the obtained star anise embryogenic cells can induce the regeneration of star anise plants, and have the advantages of fast growth speed, high plant regeneration frequency, stable properties and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapid propagation of star anise lotus. Background technique [0002] Podophyllotoxin (PTOX) is a cyclolignan lactone in lignans. It is a natural product with anti-tumor activity extracted from podophyllum plants. It is a synthetic etoposide ( The precursor of drugs such as etoposide (VP-16) and teniposide (teniposide, VM-26). Studies have shown that podophyllotoxin has special effects in the treatment of chronic diseases such as esophageal cancer and uterine cancer, and the market demand is huge. With the continuous development of new functions of podophyllotoxin and the increase of human cancer rate in recent years, its market demand will also increase rapidly. Podophyllotoxin is mainly extracted from perennial herbaceous plants of the Berberidaceae Berberidaceae, such as the genus Illicium genus, the genus Prunus genus, the genus Heliconia genus and the genus Podophy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 谭小明周雅琴余丽莹韦莹周小雷
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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