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Efficient microinjection device

A microinjection and high-efficiency technology, applied in the field of cell biology research, can solve the problems of high cost, difficult operation and complicated equipment

Active Publication Date: 2016-03-23
HOHAI UNIV CHANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this device can significantly improve the accuracy rate and reduce the damage to the cell membrane by injection, it also has problems such as complex equipment, high difficulty in operation, high cost, low efficiency and automation.

Method used

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Embodiment Construction

[0016] In order to make the content of the present invention more clearly understood, the present invention will be further described in detail below based on specific embodiments and in conjunction with the accompanying drawings.

[0017] Such as Figure 1~4 As shown, a high-efficiency microinjection device includes a quartz substrate 1 and an injection body 2, the injection body 2 is made of polydimethylsiloxane material, the injection body 2 is fixed on the quartz substrate 1, and the injection body 2 is provided with an input microchannel 60, an output microchannel 30, and a cell delivery microchannel 20 connecting the input microchannel 60 and the output microchannel 30, and the input microchannel 60 and the output microchannel are provided on the injection body 2. A microinjection flow channel 50 and a control cell movement flow channel 70 are also arranged between the 30, the microinjection flow channel 50 is in a vertical direction and communicates vertically with the ...

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Abstract

The invention discloses an efficient microinjection device which comprises a quartz substrate and an injection body. The injection body is fixed on the quartz substrate and provided with an input microchannel, an output microchannel and a cell feeding microchannel, and the portion, between the input microchannel and the output microchannel, of the injection body is provided with a microinjection channel and a cell movement control channel; the microinjection channel is arranged in the vertical direction, perpendicularly communicated with the cell feeding microchannel and fixedly provided with a microinjection needle, a gap is formed between the microinjection channel and the microinjection needle, and the size of the gap is smaller than the diameter of the cells; a limit protrusion is arranged in the microinjection channel, and the cell movement control channel is perpendicularly communicated with the microinjection channel and located over the limit protrusion. According to the efficient microinjection device, cell movement is indirectly controlled by controlling microfluid movement to enable the cells to run into the microinjection needle by themselves, and therefore microinjection operation of the cells is achieved.

Description

technical field [0001] The invention relates to a high-efficiency microinjection device, which belongs to the technical field of cell biology research. Background technique [0002] Microinjection technology is a technology that injects exogenous genes or other substances into cells by using microinjection needles after adjusting and fixing the injected cells in the field of view of the microscope. It is one of the important technical means of modern bioengineering, especially transgenic , cloning and test-tube babies and other cell engineering fields. Compared with other transgenic techniques, microinjection has the advantages of stable transgenic efficiency, unlimited injection gene size, no need for vectors, and no special requirements for operating objects, etc. been widely used in. The existing microinjection device comprises a moving part with a moving control knob, a needle holder and an injection needle connected with the moving part, and piezoelectric ceramics con...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/00
CPCC12M23/16C12M33/00C12M35/00C12M41/00
Inventor 吕品纪爱敏朱晓璐王祥冰刘源
Owner HOHAI UNIV CHANGZHOU
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