Kit for postoperative prognosis assessment of liver cancer and chemotherapy sensitizer for liver cancer
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A prognostic assessment and reagent technology, applied in the field of biomedicine, can solve the problems of no postoperative prognosis assessment kit for liver cancer, no liver cancer chemotherapy sensitizer, etc.
Active Publication Date: 2019-02-19
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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[0005] So far, there is no liver cancer postoperative prognosis assessment kit using miR-653 as a biomarker, and there is no liver cancer chemotherapy sensitizer using miR-653 as an active ingredient
Method used
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Embodiment 2
[0052] The surgical specimens of 212 cases of hepatic mass resection used in Example 2 were approved by the Medical Ethics Committee of Renmin Hospital of Wuhan University and collected from cases admitted to Renmin Hospital of Wuhan University from January 2005 to December 2013.
Embodiment 4
[0053] The nude mice / mice used in Example 4 were purchased from Hunan Slack Jingda Experimental Animal Co., Ltd.
[0056] The pSicoR vector was purchased from Youbao Biology;
[0057] miRNA mimic and transfection reagent riboFECTY TM CP was purchased from Guangzhou Ruibo Biotechnology Co., Ltd.;
[0058] The kit for detection of miRNA by reverse transcription fluorescent quantitative PCR method is All-in-One TM qPCR Mix was purchased from GeneCopoeia, Inc. company.
Embodiment 1
[0059] Embodiment 1, postoperative prognosis assessment kit for liver cancer according to the present invention and its preparation method
[0060] This example provides a kit for postoperative prognosis assessment of liver cancer. The kit specifically includes primers for PCR amplifying the cDNA of miR-653, and its upstream and downstream sequences are as follows:
[0063] Further, the kit also includes RNA extraction reagents, a buffer system, reverse transcription reagents, and PCR amplification reagents.
[0064] Further, the kit of the present invention also includes a reverse transcription primer for miR-653, as shown in Seq ID No.3.
[0065] This embodiment also provides the preparation method of the above-mentioned kit, the steps are: the reagents for assembling the kit include primers for PCR amplifying the cDNA of miR-653; The primers for PCR amplification...
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Abstract
The invention discloses a kit for postoperative and prognosis evaluation of liver cancer, and relates to the field of biological medicine. The kit comprises an agent for quantitatively detecting an expression level of micro RNA (Ribonucleic Acid) molecules miR-653 in to-be-detected liver tissues; a nucleotide sequence of the miR-653 is as shown in Seq ID No.1. Active ingredients of a liver cancer chemosensitizer comprise the mutually prepared micro RNA molecules miR-653 and a precursor of the micro RNA molecules miR-653, or an overexpression vector capable of effectively expressing the micro RNA molecules miR-653 and / or the precursor of the miR-653; the precursor of the micro RNA molecules miR-653 is encoded by a DNA (Deoxyribose Nucleic Acid) sequence as shown in Seq ID No.2. Experimental data verify the effectiveness of the kit disclosed by the invention in the aspect of postoperative and prognosis evaluation of liver cancer and verifies that the sensitizer based on the miR-653 can effectively reinforce the sensitivity of liver cancer cells for a chemotherapy drug sorafenib.
Description
technical field [0001] The invention relates to the field of biomedicine, in particular to a postoperative prognosis assessment kit for liver cancer and a chemotherapy sensitizer for liver cancer. Background technique [0002] Hepatocellular carcinoma (HCC, hereinafter referred to as liver cancer) is a malignant tumor disease that seriously threatens the life and health of our people. Worldwide, liver cancer has become the third leading cause of cancer-related death. In recent years, imaging diagnostic techniques and surgical techniques related to liver cancer have been continuously improved. Targeted therapy drugs for liver cancer represented by sorafenib have also been approved by the US Food and Drug Administration. These achievements provide more and more effective options for the diagnosis and treatment of liver cancer. But unfortunately, looking at the current clinical treatment of liver cancer, the five-year survival rate of liver cancer patients has not been signi...
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