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Application of chrysophanol or its derivatives and oncolytic virus in the preparation of antitumor drugs

A technology of oncolytic virus and chrysophanol, which is applied in the field of biomedicine to achieve the effects of enhancing inhibition, increasing anti-tumor effect, and improving therapeutic effectiveness

Active Publication Date: 2019-09-24
GUANGZHOU VIROTECH PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the M1 antitumor synergist

Method used

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  • Application of chrysophanol or its derivatives and oncolytic virus in the preparation of antitumor drugs
  • Application of chrysophanol or its derivatives and oncolytic virus in the preparation of antitumor drugs
  • Application of chrysophanol or its derivatives and oncolytic virus in the preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Chrysophanol and M1 virus significantly increase the morphological lesions of human hepatocellular carcinoma lines

[0041] Material:

[0042] Human hepatocellular carcinoma Hep3B, M1 virus, high glucose DMEM medium, inverted phase contrast microscope.

[0043] method:

[0044]a) Cell culture: Human hepatocellular carcinoma Hep3B was grown in DMEM complete medium containing 10% FBS, 100 U / ml penicillin and 0.1 mg / ml streptomycin; all cell lines were placed in 5% CO 2 , cultured and subcultured in a closed incubator with a constant temperature of 37°C (95% relative humidity), and observed the growth with an inverted microscope. The cells were subcultured every 2-3 days, and the cells in the logarithmic growth phase were taken for formal experiments.

[0045] b) Cell treatment and morphological observation: cells in the logarithmic growth phase were selected, and DMEM complete culture medium (containing 10% fetal bovine serum, 1% double antibody) was used to ...

Embodiment 2

[0048] Example 2 Combined treatment of chrysophanol and M1 virus significantly reduced the survival rate of human hepatocellular carcinoma lines

[0049] Material:

[0050] Human hepatocellular carcinoma Hep3B, M1 virus, high glucose DMEM medium, automatic enzyme-linked detection microplate reader.

[0051] method:

[0052] a) Cell inoculation, administration treatment: select logarithmic growth phase cells, DMEM complete culture medium (containing 10% fetal calf serum, 1% double antibody) to make cell suspension, with 4 × 10 3 The density per well was seeded in a 96-well culture plate. After 12 hours, the cells were completely attached to the wall. The experiments were divided into control group, chrysophanol alone group, M1 infection group and chrysophanol / M1 combined group. The doses used were: M1 virus (MOI=0.001) infected cells; different dose gradients were set for chrysophanol.

[0053] b) Reaction of MTT with intracellular succinate dehydrogenase: when cultured for...

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Abstract

The present invention relates to the field of biological medicine and discloses an application of chrysophanol or its derivatives and oncolytic virus in preparing antitumor drugs. It is found for the first time that the chrysophanol or its derivatives can be used to prepare a synergistic agent with oncolytic virus in terms of anti-tumor effects. Also disclosed are a pharmaceutical composition comprising chrysophanol and oncolytic virus, a pharmaceutical package comprising chrysophanol and oncolytic virus, and the use of chrysophanol and oncolytic virus in treating tumors, in particular tumors insensitive to oncolytic virus.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of the combination of chrysophanol and oncolytic virus in the preparation of antitumor drugs. Background technique [0002] Oncolytic virus is a kind of replication-competent virus that targets and infects and kills tumor cells without destroying normal cells. Oncolytic virus therapy (oncolytic virotherapy) is an innovative tumor-targeted treatment strategy, which uses natural or genetically engineered viruses to selectively infect tumor cells and replicate in tumor cells to achieve targeted dissolution, The effect of killing tumor cells, but harmless to normal cells. [0003] M1 virus (Alphavirus M1) belongs to the genus Alphavirus (Alphavirus), and its application in the preparation of anti-tumor drugs has been recorded in Chinese invention patent application 201410425510.3. The M1 virus can selectively cause the death of tumor cells without affecting the survival of n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K31/122
Inventor 颜光美张海鹏高光坪程世源毛子旭郑明豪林穗珍龚守芳胡骏朱文博银巍
Owner GUANGZHOU VIROTECH PHARMA
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