Efficient culture medium as well as preparation method and application thereof

A culture medium and high-efficiency technology, applied in the biological field, can solve the problems of limited increase in fermentation titer, lack of specific elements, precursors and promoters in the culture medium, and achieve suppression of by-product formation, improvement of fermentation titer, and economic benefits. significant effect

Inactive Publication Date: 2016-04-06
青岛海科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The culture medium of the above two inventions lacks specific elements, precursors and accelerators,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] The high-efficiency culture medium provided in this embodiment is composed of the following components: soybean meal powder, yeast powder, cottonseed protein peptide, starch, sodium dihydrogen phosphate, sodium bromide, acetyl coenzyme A, and rare earth nitrate.

[0025] Wherein, the rare earth nitrate is cerium nitrate.

[0026] Among them, the mass ratio of soybean meal powder, yeast powder, cottonseed protein peptide, starch, and sodium dihydrogen phosphate in the medium is 3.5:1.5:1.5:5:0.05.

[0027] Among them, the weight content of sodium bromide in the culture medium is 0.08%, the weight content of acetyl-CoA is 0.06%, and the weight content of rare earth nitrate is 0.03%.

[0028] Among them, the protein content of soybean meal powder is 40-55%, the protein content of yeast powder is 45-55%, and the protein content of cottonseed protein peptide is 55-70%.

[0029] Among them, the cottonseed protein peptide is produced by enzymatic hydrolysis, and the en...

Example Embodiment

[0031] Example 2

[0032] The difference between this embodiment and Embodiment 1 is: (1) The rare earth nitrate uses praseodymium nitrate. (2) The mass ratio of soybean meal powder, yeast powder, cottonseed protein peptide, starch, and sodium dihydrogen phosphate in the medium is 3:1.5:1.5:5:0.05. (3) The weight content of sodium bromide in the culture medium is 0.02%, the weight content of acetyl-CoA is 0.02%, and the weight content of rare earth nitrate is 0.03%.

Example Embodiment

[0033] Example 3

[0034] The difference between this embodiment and Embodiment 1 lies in: (1) Neodymium nitrate is used as the rare earth nitrate. (2) The mass ratio of soybean meal powder, yeast powder, cottonseed protein peptide, starch, and sodium dihydrogen phosphate in the medium is 4:1.5:1.5:5:0.05. (3) The weight content of sodium bromide in the culture medium is 0.1%, the weight content of acetyl-CoA is 0.06%, and the weight content of rare earth nitrate is 0.03%.

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PUM

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Abstract

The invention provides an efficient culture medium as well as a preparation method and application thereof. The efficient culture medium is prepared mainly by culture medium component optimization and process improvement. The main raw materials of the culture medium mainly comprises the substances of yeast powder, cottonseed protein peptide, soybean cake powder, starch, sodium dihydrogen phosphate, sodium bromide, acetyl coenzyme A, rare earth element and the like, and the efficient culture medium is prepared by fully mixing the substances and making into micro powder. The efficient culture medium contains nutrient substances and microelement required by the growth of thalli, and specific precursors and accelerants, is capable of effectively promoting the production of a target product and inhibiting the generation of a by-product, and is especially suitable for tetracycline fermentation, and the yield and purity of a fermented product are high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-efficiency culture medium and its preparation method and application. Background technique [0002] my country is the world's main producer of pharmaceutical raw materials, and antibiotics, amino acids, vitamins and other products occupy an important position in the world market. The total annual production of antibiotic raw materials in my country is about 50,000 tons. Most of these antibiotic raw materials are produced by industrial fermentation, and the total demand for medium is more than 200,000 tons. The output of my country's three major fermentation products is about 1 million tons, and the fermentation production of enzyme preparations such as amylase, protease, cellulase, and glucoamylase requires a large amount of culture medium. The production capacity of amino acids continues to expand, and the demand for culture media is also growing rapidly. [000...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P29/00C12R1/49
CPCC12N1/20C12P29/00
Inventor 张术臻梁小田李俊芳
Owner 青岛海科生物技术有限公司
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