A white light coaxial interference phase-shift microscope system and microscopic imaging method
A microscope system and interferometric phase shift technology, which is applied in the field of white light coaxial interferometric phase shift microscope system, can solve the problem of low interference modulation degree and achieve the effect of reducing costs
Active Publication Date: 2017-12-15
SOUTH CHINA NORMAL UNIVERSITY
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[0005] The purpose of the present invention is to solve the problem of low interference modulation in the white light coaxial interference phase-shift microscope system
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[0022] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.
[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terminology used herein in the description of the present invention is only for the purpose of describing specific embodiments, and is not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of th...
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The invention provides a white light coaxial interference phase shift microscope system, which comprises a microscope device and a phase imaging device, wherein the phase imaging device comprises a light source, a first lens, a transmission-type pure amplitude spatial light modulator, a second lens, a third lens, a reflection-type pure phase spatial light modulator, a fourth lens and a camera, wherein the transmission-type pure amplitude spatial light modulator is loaded with a pattern matched with the frequency spectrum of an image for reducing light intensity of low frequency information to enable the light intensity of the low frequency information to be equal with that of high frequency information; the incidence angle of the reflection-type pure phase spatial light modulator is smaller than 10 DEG; and the reflection-type pure phase spatial light modulator is loaded with multiple patterns with different phase shift amounts to modulate the low frequency information. The invention also provides a microscopic imaging method based on the white light coaxial interference phase shift microscope system.
Description
technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to a white light coaxial interference phase-shift microscope system and a microscopic imaging method. Background technique [0002] Ordinary microscope imaging only allows the amplitude change (brightness) and wavelength change (color) of light to be observed, but most living tiny organisms are colorless and transparent. It is difficult to observe clearly under the field microscope. In order to overcome this difficulty, measures such as dyeing have been adopted to change the color and brightness of the object to be inspected. However, this method is mostly used in a non-living state, and the effect is not particularly ideal when applied to a living body. Of course, the aperture stop of the condenser is narrowed to increase the contrast between light and dark, but such fine structures are difficult to be resolved, and the brightness decreases accordingly. [0003] In r...
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IPC IPC(8): G02B21/00G02B21/36G02B21/02G02B21/06
CPCG02B21/0004G02B21/02G02B21/06G02B21/36
Inventor 王翰林吕晓旭钟丽云
Owner SOUTH CHINA NORMAL UNIVERSITY